Keywords: ATD; arrival time distribution; CCS; collision cross section; CID; collision induced dissociation; CIU; collision induced unfolding; DDM; n-dodecyl-β-D-maltoside; ESI; electrospray ionization; FPOP; fast photochemical oxidation of proteins; HDX; hydrogen
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Semi-automated screen for global protein conformational changes in solution by ion mobility spectrometry-massspectrometry combined with size-exclusion chromatography and differential hydrogen-deuterium exchange
Keywords: Protein folding; SEC; HDX; IMS; Global conformational change;
Keywords: SEC; Protein in solution; HDX; UHPLC; Chaotropic additive; Global conformational changes;
Keywords: PR; progesterone receptor; SHR; steroid hormone receptor; CoR; co-regulatory protein; LBD; ligand-binding domain; DBD; DNA-binding domain; NTD; amino-terminal domain; ID; intrinsically disordered; AF; activation function; HDX; hydrogen-deuterium exchang
Keywords: ESI; electrospray ionization; SLD; soft laser desorption; MALDI; matrix-assisted laser desorption/ionization; ICAT; isotope-coded affinity tags; SILAC; stable isotope labeling by amino acids in cell culture; TMT; tandem mass tags; iTRAQ; isobaric tags for
Keywords: AF-2; activation function 2; AP; alternative pocket; AR; androgen receptor; BF-3; binding function 3; DBD; DNA-binding domain; ER; estrogen receptor; GP; genomic pocket; GR; glucocorticoid receptor; H12; helix 12; HDX; hydrogen/deuterium exchange; LBD; li
Keywords: HDX; hydrogen/deuterium exchange; MS; mass spectrometry; TCEP; tris(2-carboxyethyl)phosphine; LC; liquid chromatography; HDX/MS; Insulin; Insulin lispro;
Keywords: CCS; collision cross-section; ESI-MS; electrospray ionization-mass spectrometry; HDX; hydrogen-deuterium exchange; IMS-MS; ion mobility spectrometry-mass spectrometry; Amyloid; Ion mobility spectrometry; Mass spectrometry; Oligomer; Ligand bindi
Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator
Keywords: enChIP; HIF-2α; HDX; MYCN; Hypoxia; Neuroblastoma;
Installation, validation, and application examples of two instrumental setups for gas-phase HDX-MS analysis of peptides and proteins
Keywords: HDX; hydrogen/deuterium exchange; HDX-MS; hydrogen/deuterium exchange coupled to mass spectrometry; ND3; deuterated ammonia; pND3; partial pressure of ND3; TWIG; traveling wave ion guide; Gas-phase hydrogen/deuterium exchange mass spectrometry; Gas-phase
Chicken GRIFIN: Structural characterization in crystals and in solution
Keywords: Aggregation; Crystallin; Galectin; Lectin; Lens; Ultracentrifugation; Ã
; Ã
ngström; Arg; arginine; Asn; asparagine; Asp; aspartate; CG; chicken galectin; C-GRIFIN; chicken galectin-related inter-fiber protein; Gln; glutamine; Glu; glutamate; HDX; hydrog
Monitoring of the functional properties and unfolding change of Ovalbumin after DHPM treatment by HDX and FTICR MS: Functionality and unfolding of Oval after DHPM by HDX and FTICR MS
Keywords: DHPM; dynamic high pressure microfluidization; HDX; hydrogen-deuterium exchange; FTICR MS; Fourier-transform ion cyclotron resonance mass spectrometry; FC; foaming capacity; FS; foaming stability; EAI; emulsifying activity index; ESI; emulsion stability
Impurity profiling of liothyronine sodium by means of reversed phase HPLC, high resolution mass spectrometry, on-line H/D exchange and UV/Vis absorption
Keywords: Liothyronine; LT3Na; Liothyronine sodium; HRMS; HRMS/MS; Impurity profiling; HDX; H/D exchange; API analysis; Thyroxine; Hypothyroidism; Hormone deficiency; UV Vis spectroscopy;
Levothyroxine sodium revisited: A wholistic structural elucidation approach of new impurities via HPLC-HRMS/MS, on-line H/D exchange, NMR spectroscopy and chemical synthesis
Keywords: Levothyroxine; Thyroid hormone; Pharmaceutical impurities; On-line H/D Exchange; HDX; LC-MS;
Identification and Structural Characterization of the Precursor Conformation of the Prion Protein which Directly Initiates Misfolding and Oligomerization
Keywords: PrP; prion protein; PrPC; cellular PrP; PrPSc; scrapie PrP; HDX; hydrogen-deuterium exchange; moPrP; mouse PrP; PUF; partially unfolded form; CTD; C-terminal domain; dm CTD; double mutant variant of the C-terminal domain; wt; wild-type; DLS; dynamic lig
The excluded DNA strand is SEW important for hexameric helicase unwinding
Keywords: Helicase; Unwinding; Steric exclusion; Hydrogen-deuterium mass spectrometry; Single molecule FRET; DNA damage sensing; AAA+; ATPases associated with various activities; ARDD; average relative deuterium uptake; BER; base excision repair; BrdU; bromodeoxyur
Structural Dynamics in Ras and Related Proteins upon Nucleotide Switching
Keywords: MD; molecular dynamics; HDX; hydrogen-deuterium exchange; MS; mass spectrometry; P-loop; phosphate-binding loop; GDP; guanosine diphosphate; GTP; guanosine triphosphate; GAP; GTPase-activating protein; SIIP; switch II pocket; GMPPNP; guanylyl imidodipho
Steric gate residues of Y-family DNA polymerases DinB and pol kappa are crucial for dNTP-induced conformational change
Keywords: dNTP; deoxyribonucleoside triphosphate; rNTP; ribonucleoside triphosphate; pol; polymerase; HDX; hydrogen/deuterium exchange; MS; mass spectrometry; LF; little finger; WT; wild-type; DNA replication; Nucleotide selection; Enzyme specificity; Ribonucleotid
Ion molecule reaction H/D exchange as a probe for isomeric fractionation in chromatographically separated natural organic matter
Keywords: D0; parent ion, containing no deuterium; DBE; double bond equivalency (number of rings and double bonds); Dmax; maximum number of H/D exchanges observed for any given ion; f*; family score (distinguished between families of isobars in NOM); HDX; hydrogen/
Conformational dynamics of human FXR-LBD ligand interactions studied by hydrogen/deuterium exchange mass spectrometry: Insights into the antagonism of the hypolipidemic agent Z-guggulsterone
Keywords: HDX; hydrogen/deuterium exchange; LC-ESI-MS; liquid chromatography electrospray ionization mass spectrometry; FXR-LBD; farnesoid X receptor ligand binding domain; LBC; ligand binding cavity; CDCA; chenodeoxycholic acid; GG; Z-guggulsterone; 6ECDCA; 6-et
Structural basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1
Keywords: HDX; H/D exchange; HDX-MS; H/D exchange coupled to mass spectrometry; DSS; disuccinimidyl suberate; DSG; disuccinimidyl glutarate; WT; wild type; Nth1; neutral trehalase; pNth1; phosphorylated neutral trehalase; DMSO; dimethyl sulfoxide; TCEP; tris (2-c
Top-down hydrogen/deuterium exchange and ECD-stitched FTICR-MS for probing structural dynamics of a 29-kDa enzyme
Keywords: Top-down; Hydrogen/deuterium exchange; HDX; Electron capture dissociation; ECD; Structural dynamics; Drug binding; Conformational change
Transferrin as a model system for method development to study structure, dynamics and interactions of metalloproteins using mass spectrometry
Keywords: BBB; blood-brain barrier; ESI; electrospray ionization; FAB; fast atom bombardment; Fe2hTf; diferric form of human serum transferrin; GHT; human growth hormone-human serum transferrin fusion protein; HDX; hydrogen/deuterium exchange; hTf; human serum tr
Changes in solvent accessibility of wild-type and deamidated βB2-crystallin following complex formation with αA-crystallin
Keywords: lens; crystallins; age-related modifications; cataracts; deamdidation; alpha-crystallin; beta-crystallins; C-td; C-terminal domain; DM; double mutant; HDX; hydrogen-deuterium exchange; MALS; multi-angle light scattering; N-td; N-terminal domain; SEC; si
Application of micro-reactor chip technique for millisecond quenching of deuterium incorporation into 70S ribosomal protein complex
Keywords: HDX; hydrogen/deuterium exchange; MALDI; matrix-assisted laser desorption/ionization; MS; mass spectrometry; TOF; time of flight; H/D exchange; Ribosome; dynamics; micro-reactor; rapid quenching;
EX1 hydrogen-deuterium exchange in an all-helical protein and its cyclized derivative at neutral pH
Keywords: HDX; hydrogen-deuterium exchange; NH4OAc; ammonium acetate; ESI; electrospray ionization; NMR; nuclear magnetic resonance (spectrometry); Hydrogen-deuterium exchange; EX1 limit; Electrospray ionization mass spectrometry; ESI-MS;
Hydrogen/deuterium exchange mass spectrometry and optical spectroscopy as complementary tools for studying the structure and dynamics of a membrane protein
Keywords: AFM; atomic force microscope; BO; bacterioopsin (BR without retinal chromophore); BR; bacteriorhodopsin; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; CMC; critical micelle concentration; DM; n-dodecyl-d-maltoside; DMPC; 1,2-dimyristoy
Methods for the analysis of high precision differential hydrogen-deuterium exchange data
Keywords: HDX; MS; Ligand; Screening; Nuclear receptor;
A comparison of mass spectrometry based hydrogen deuterium exchange methods for probing the cyclophilin A cyclosporin complex
Keywords: HDX; Cyclosporin; Cyclophilin A; PLIMSTEX; SUPREX; Gas phase HDX