دانلود مقالات ISI درباره Isopropyl-Β-D-Thiogalactoside + ترجمه فارسی

بیوسنتز و مشخص کردن یک پلی پپتیدی غیر تکراری از زنجیره سنگین فیبرین ابریشمی

Biosynthesis and characterization of a non-repetitive polypeptide derived from silk fibroin heavy chain

Keywords: IPTG; isopropyl-β-d-thiogalactoside; SDS; sodium dodecyl sulfate; PAGE; polyacrylamide gel electrophoresis; GST; glutathione S-transferase; f(4); four repeats of designed gene motif f(1); f(8); eight repeats of designed gene motif f(1); F(1); expression

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2016 سفارش ترجمه

تعیین کننده های مولکولی تشخیص استاف الیوپریستال توسط گیرنده های اکسستروئید

Molecular determinants of the recognition of ulipristal acetate by oxo-steroid receptors

Keywords: AR; androgen receptor; DMEM; Dulbecco's minimal essential medium; FCS; fetal calf serum; GR; glucocorticoid receptor; GRE; glucocorticoid response element; GST; glutathione S-transferase; HEK; human embryonic kidney; IPTG; isopropyl-β-d-thiogalactoside

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2014 سفارش ترجمه

پروتئین تت گلیکسالاز مانع آسیب سلول های عصبی ایسکمیک می شود و آسیب های ایسکمیک را کاهش می دهد

Tat-glyoxalase protein inhibits against ischemic neuronal cell damage and ameliorates ischemic injury

Keywords: AGE; glycation end products; CV; cresyl violet; DAPI; 4′,6-diamidino-2-phenylindole; DCF-DA; 2′,7′-dichlorofluorescein diacetate; F-JB; Fluoro-Jade B; GLO; glyoxalase; IPTG; isopropyl-β-D-thiogalactoside; JNK; c-Jun N-terminal kinase; MAPK; mitogen

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2014 سفارش ترجمه

Autoubiquitination of feline E3 ubiquitin ligase BCA2

Keywords: BCA2; breast cancer associated gene 2; RNF115; ring finger protein 115; BZF; BCA2 zinc finger; Mdm2; mouse double minute 2; BRCA1; breast cancer 1; FMC; feline mammary carcinoma; WHO; World Health Organization; ATCC; American Type Culture Collection; BCIP

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2018 سفارش ترجمه

Cloning and characterization of a novel phenylalanine ammonia-lyase gene from Inonotus baumii

Keywords: HPLC; high-performance liquid chromatography; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; DMW; dry mycelium weight; DOP-PCR; degenerate oligonucleotide primer PCR; RACE-PCR; rapid-amplification of cDNA ends PCR; vgb; Vitreoscilla

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2018 سفارش ترجمه

Molecular characterization and in silico analysis of ectoine biosynthesis genes from the deep sea halophilic eubacteria, Bacillus clausii NIOT-DSB04

Keywords: ectA, B & C; ectoine biosynthesis genes; IPTG; isopropyl-β-d-thiogalactoside; PDB; Protein Data Bank; Bacillus clausii; Halophilic bacteria; Compatible solutes; Ectoine genes; Osmoregulation;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2018 سفارش ترجمه

The N-terminal domains of Bacillus subtilis CopA do not form a stable complex in the absence of their inter-domain linker

Keywords: CD; Circular dichroism; DTNB; 5,5′-dithiobis-(2-nitrobenzoic acid); DTT; dithiothreitol; ESI-MS; electrospray ionization mass spectrometry; Hepes; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HSQC; heteronuclear single quantum correlation; ICP-AE

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2018 سفارش ترجمه

Three novel variants (p.Glu178Lys, p.Val245Met, p.Ser250Phe) of the phenylalanine hydroxylase (PAH) gene impair protein expression and function in vitro

Keywords: BH4; Tetrahydrobiopterin; E. coli; Escherichia coli; ELISA; Enzyme-linked immunosorbent assay; hPAH; Human phenylalanine hydroxylase; IPTG; Isopropyl-β-d-thiogalactoside; kDa; Kilo Dalton; MBP; Maltose binding protein; PAH; Phenylalanine hydroxylase prot

دانلود رایگان متن کامل مقاله ISI 22 صفحه سال انتشار : 2018 سفارش ترجمه

Probing the structure-function relationship of Mycobacterium leprae HSP18 under different UV radiations

Keywords: sHSP; small heat shock protein; DTT; dithiothreitol; IPTG; isopropyl-β-d-thiogalactoside; bis-ANS; 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt; NFK; N-formyl kynurenine; Kyn; Kynurenine; Mycobacterium leprae HSP18; Small h

دانلود رایگان متن کامل مقاله ISI 48 صفحه سال انتشار : 2018 سفارش ترجمه

TsPKA-r: a potential immunodiagnostic antigen for the detection of porcine cysticercosis

Keywords: PKA; cAMP-dependent protein kinase; bp; base pair; PCR; polymerase chain reaction; iELISA; indirect enzyme-linked immunosorbent assays; LB; Luria-Bertani; IPTG; isopropyl-β-d-thiogalactoside; BCA; bicinchonininc acid; TMB; 3,3',5,5'-tetramethylbenzid

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2017 سفارش ترجمه

Identification of sennoside A as a novel inhibitor of the slingshot (SSH) family proteins related to cancer metastasis

Keywords: PTPs; protein tyrosine phosphatases; PTKs; protein tyrosine kinases; DUSP; dual specificity phosphatase; ADF; actin-depolymerizing factor; IPTG; isopropyl-β-d-thiogalactoside; DiFMUP; 6,8-difluoro-4-methylumbelliferyl phosphate; MBP; maltose binding prot

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2017 سفارش ترجمه

Use of polymer conjugates for the intraperoxisomal delivery of engineered human alanine:glyoxylate aminotransferase as a protein therapy for primary hyperoxaluria type I

Keywords: Protein therapeutics; Polymer conjugates; Primary hyperoxaluria; Rare disease; Peroxisomal targeting; Enzyme administration; PH1; primary hyperoxaluria type I; AGT; alanine:glyoxylate aminotransferase; PLP; pyridoxal 5′-phosphate; PTS; peroxisomal targe

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2017 سفارش ترجمه

Plant E3 ligases ubiquitinate Escherichia coli σ32in vitro

Keywords: E3 ligase; ubiquitin; σ32; Escherichia coli; IPTG; isopropyl-β-d-thiogalactoside; CBB; coomassie Brilliant Blue; Co-IP; Co-Immunoprecipitation; UBL; ubiquitin-like protein; HSR; heat shock response;

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2017 سفارش ترجمه

Bombyx mori Serpin6 regulates prophenoloxidase activity and the expression of antimicrobial proteins

Keywords: Bmserpin6; Bombyx mori serpin6; PBST; Phosphate buffered saline containing 0.02% tween-20; PPO; prophenoloxidase; AMPs; antimicrobial peptides; RT-qPCR; Real-time quantitative PCR; Mi; Micrococcus luteus; Sm; Serratia marcescens; SilkDB; silkworm genome d

دانلود رایگان متن کامل مقاله ISI 21 صفحه سال انتشار : 2017 سفارش ترجمه

Analysis of pupylation of Streptomyces hygroscopicus 5008 in vitro

Keywords: Pup; Streptomyces hygroscopicus 5008; Pupylation; Dop; IPTG; isopropyl-β-d-thiogalactoside; CBB; coomassie Brilliant Blue; S. hygroscopicus; Streptomyces hygroscopicus 5008;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2016 سفارش ترجمه

Cloning and characterization of a robust recombinant azoreductase from Shewanella xiamenensis BC01

Keywords: Azoreductase; Recombination; Molecular docking; Shewanella xiamenensis; Solvent tolerance; BSA; bovine serum albumin; DMF; dimethyl formamide; DMSO; dimethyl sulfoxide; IAA; iodoacetamide; IPTG; isopropyl-β-D-thiogalactoside; POE-PCR; prolonged overlap e

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2016 سفارش ترجمه

Overexpression of lycopene ε-cyclase gene from lycium chinense confers tolerance to chilling stress in Arabidopsis thaliana

Keywords: ROS; reactive oxygen species; ORF; open reading frame; SOD; superoxide dismutase; POD; peroxidase; MDA; malondialdehyde; PSII; photosystem II; NPQ; Non-photochemical quenching; OD; optical density; IPTG; isopropyl-β-D-thiogalactoside; SDS-PAGE; sodium do

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2016 سفارش ترجمه

Heat shock transcription factor δ32 is targeted for degradation via an ubiquitin-like protein ThiS in Escherichia coli

Keywords: δ32; Escherichia coli; Ubiquitin-like protein; ThiS; IPTG; isopropyl-β-d-thiogalactoside; UPS; ubiquitin-proteasome system; UBLs; ubiquitin-like proteins; HSR; heat shock response; CBB; coomassie Brilliant Blue; Co-IP; Co-Immunoprecipitation.;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2015 سفارش ترجمه

A rice DEAD-box RNA helicase protein, OsRH17, suppresses 16S ribosomal RNA maturation in Escherichia coli

Keywords: 2,4-D; 2,4-dichlorophenoxyacetic; NAA; naphthyl acetic acid; 6-BA; 6-benzyladenine; ABA; abscisic acid; E. coli; Escherichia coli; GA; gibberellin; GFP; green fluorescent protein; GUS; β-glucuronidase; IPTG; isopropyl-β-d-thiogalactoside; KT; kinetin; S

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2015 سفارش ترجمه

SPR analysis of promoter binding of Synechocystis PCC6803 transcription factors NtcA and CRP suggests cross-talk and sheds light on regulation by effector molecules

Keywords: NtcA; nitrogen control factor of cyanobacteria; CRP; cAMP receptor protein, also called CAP, catabolite gene activator protein; PipX; PII-interacting protein X; SYCRP1; Synechocystis CRP; 2OG; 2-oxoglutarate; RU; relative unit; SPR; surface plasmon resona

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

The crystal structure of ferritin from Chlorobium tepidum reveals a new conformation of the 4-fold channel for this protein family

Keywords: Crystal structure; Bacterial ferritin; Ferroxidase center; HAADF-STEM; HADDF-STEM; high angle annular dark field microscopy; IPTG; isopropyl-β-d-thiogalactoside; MOPS; 3-(N-morpholino)propane sulfonic acid; PMSF; phenylmethane sulfonyl fluoride; TEM; tra

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2014 سفارش ترجمه

Lethal protein in mass consumption edible mushroom Agrocybe aegerita linked to strong hepatic toxicity

Keywords: Agrocybe aegerita; Hepatotoxicity; Lectin AAL; Stability; Total protein; Yt; total components; Yp; total protein compounds; Ys; small molecule compounds; AAL; Agrocybe aegerita lectin; ALT; serum alanine aminotransferase; AST; serum aspartate aminotransfe

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2014 سفارش ترجمه

GRP78 secreted by tumor cells stimulates differentiation of bone marrow mesenchymal stem cells to cancer-associated fibroblasts

Keywords: CAFs; cancer-associated fibroblasts; MSCs; mesenchymal stem cells; ER; endoplasmic reticulum; α-SMA; α-smooth muscle actin; HBMSCs; human bone marrow mesenchymal stem cells; BMMSCs; murine bone marrow mesenchymal stem cells; SDF-1; stromal cell-derived

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Design and characterization of a recombinant colorimetric SAG1-alkaline phosphatase conjugate to detect specific antibody responses against Toxoplasma gondii

Keywords: T. gondii; Toxoplasma gondii; SAG1; surface antigen-1; AP; alkaline phosphatase; IPTG; isopropyl-β-d-thiogalactoside; PBS; phosphate-buffered saline; NBT; nitro-blue tetrazolium; BCIP; 5-bromo-4-choro-1-indolylphosphate; SDS-PAGE; sodium dodecyl sulfate

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Trp266 determines the binding specificity of a porcine aflatoxin B1 aldehyde reductase for aflatoxin B1-dialdehyde

Keywords: AFB1; aflatoxin B1; AFG1; aflatoxin G1; AFAR; aflatoxin B1 aldehyde reductase; AKR; aldo-keto reductase; AFBO; AFB1 exo-8,9-epoxide; 2-CBA; 2-carboxybenzaldehyde; SSA; succinic semialdehyde; WT; wide-type; IPTG; isopropyl-β-d-thiogalactoside; HPLC; high

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations

Keywords: ADSL; adenylosuccinate lyase; WT; wild type; SAICAR; succinylaminoimidazolecarboxamide ribonucleotide; AICAR; aminoimidazolecarboxamide ribotide; SAMP; succinyladenosine monophosphate; AMP; adenosine monophosphate; PMR; psychomotor retardation; SAICAr; su

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Expression and one-step purification of a β-galactosidase by fusion with elastin-like polypetides

Keywords: ELPs; elastin like polypeptides; RDL; recursive directional ligation; ITC; inverse transition cycling; IPTG; isopropyl-β-d-thiogalactoside; Trx; thioredoxin; CAT; chloramphenicol acetyltransferase; BFP; blue fluorescent protein; GFP; green fluorescent pr

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter

Keywords: His-tag; polyhistidine tag; IC50; half maximum inhibitory concentration; IPTG; isopropyl-β-d-thiogalactoside; LB; Luria-Bertani growth media; DDM; n-dodecyl-β-d-maltoside; Ni-NTA; nickel-nitrilotriacetic acid; ORF; open reading frame; SDS-PAGE; sodium d

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

The role of Cys271 in conformational changes of arginine kinase

Keywords: WT-AK; wild-type arginine kinase; CK; creatine kinase; Cys271; cysteine of residue-271; SEC; size exclusion chromatography; TSAC; transition state analog complex; IPTG; isopropyl-β-d-thiogalactoside; ANS; 1-anilinonaphtalene-8-sulfonate; Arginine kinase;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2011 سفارش ترجمه

Passive immunoprotection targeting a secreted CAMP factor of Propionibacterium acnes as a novel immunotherapeutic for acne vulgaris

Keywords: Acne vulgaris; Agroinfiltration; Passive immunization; Propionibacterium acnes; Radish leaves; ATCC; American Type Culture Collection; CAMP factor; Christie, Atkins, Munch-Peterson factor; CFU; Colony forming unit; ddH2O; Didistilled water; E. coli; Esche

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2011 سفارش ترجمه

Ubiquitination of LHY by SINAT5 regulates flowering time and is inhibited by DET1

Keywords: E1; ubiquitin-activating enzyme; E2; ubiquitin-conjugating enzyme; E3; ubiquitin ligase; HECT; E6-associated protein carboxyl terminus; SINA; seven in absentia; SINAT5; an Arabidopsis homologue of the SINA; Siah1; a human homologue of the SINA; NAC1; NOAP

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2010 سفارش ترجمه

Gene transcript accumulation, tissue and subcellular localization of anthocyanidin synthase (ANS) in developing grape berries

Keywords: ANR; anthocyanidin reductase; ANS; anthocyanidin synthase; BS; blocking solution; BSA; bovine serum albumin; CHS; chalcone synthase; CHI; chalcone isomerase; C4H; cinnamate-4-hydroxylase; 4CL; 4-coumarate:CoA ligase; DAPI; 4′,6′-diamidino-2-phenylindo

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2010 سفارش ترجمه

Palmitoylation modification of Gαo depresses its susceptibility to GAP-43 activation

Keywords: GAP-43; growth associated protein-43; Gαo; α subunit of heterotrimeric Go protein; GTPγS; guanosine-5′-O-(3-thiotriphosphate); p-PDM; N,N′-1,4-phenylenedimaleimide; IPTG; isopropyl-β-d-thiogalactoside; GAP-43; Gαo; Palmitoylation; GTPγS binding

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2009 سفارش ترجمه

Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis

Keywords: CPB; cysteine proteinase class B; CPB2.3; cysteine proteinase derived from the 2.3 kb DNA fragment; CPB2.3ΔCTE; CPB2.3 lacking the C-terminal extension; Abz; ortho-aminobenzoic acid; EDDnp; N-(ethylenediamine)-2,4-dinitrophenyl amide; Fmoc; fluoren-9-yl

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2008 سفارش ترجمه

Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens

Keywords: Culex pipiens pallens; RPL39; ribosomal protein L39; RT-PCR; reverse transcription polymerase chain reaction; PCR; polymerase chain reaction; 3H-TdR; methyl tritiated thymidine; cDNA; complementary deoxyribonucleic acid; RACE; rapid amplification of cDNA

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2007 سفارش ترجمه

Heterologous expression and biochemical characterization of two calcium-dependent protein kinase isoforms CaCPK1 and CaCPK2 from chickpea

Keywords: Biochemical and kinetic properties; Calcium-dependent protein kinase isoforms; Chickpea (Cicer arietinum); cDNA cloning; Recombinant expression; CaM; calmodulin; CCaMK; calcium and calmodulin-dependent protein kinase; CaCPK; Cicer arietinum CPK; CPK or CD

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2006 سفارش ترجمه

Two consecutive aspartic acid residues conferring herbicide resistance in tobacco acetohydroxy acid synthase

Keywords: AHAS; Acetohydroxy acid synthase; mAHAS; Mutant AHAS; wAHAS; Wild type AHAS; CD; Circular dichroism; FAD; Flavine adenine dinucleotide; GSH; Glutathione; GST; Glutathione S-transferase; IPTG; Isopropyl-β-d-thiogalactoside; PCR; Polymerase chain reaction;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2005 سفارش ترجمه

High-level production of human collagen prolyl 4-hydroxylase in Escherichia coli

Keywords: C-P4H; collagen prolyl 4-hydroxylase; PDI; protein disulfide isomerase; BiP; immunoglobulin heavy chain binding protein; ER; endoplasmic reticulum; aTc; anhydrotetracycline; IPTG; isopropyl-β-D-thiogalactoside; DTT; dithiothreitol; Collagen; Prolyl 4-hyd

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2005 سفارش ترجمه

Cloning and biochemical properties of CDPK gene OsCDPK14 from rice

Keywords: Calcium-dependent protein kinase (CDPK); cDNA cloning; Kinase activities; OsCDPK14; Rice (Oryza sativa); Subcellular localization; CDPK; calcium-dependent protein kinase; EDTA; ethylene diamine tetraacetic acid; EGTA; ethylene glycol-bis (β-aminoethyl et

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2005 سفارش ترجمه

The conserved residue Phe273(282) of PPARα(γ), beyond the ligand-binding site, functions in binding affinity through solvation effect

Keywords: Peroxisome proliferator-activated receptor (PPAR); Mutation; Ligand-binding affinity; Solvation effect; Molecular dynamics; Surface plamon resonance (SPR); Transcriptional activation; PPAR; peroxisome proliferator-activated receptor; KD; equilibrium disso

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2005 سفارش ترجمه

Occludin: Structure, function and regulation

Keywords: TJ; tight junction; ZO; zonula occludens; mAb; monoclonal antibody; BRB; blood-retinal barrier; TER; transepithelial/endothelial electrical resistance; IPTG; isopropyl-β-d-thiogalactoside; RT-PCR; reverse transcription-polymerase chain reaction; bp; ba

دانلود رایگان متن کامل مقاله ISI 35 صفحه سال انتشار : 2005 سفارش ترجمه