Keywords: CalB; Candida antarctica lipase B; CECF; continuous exchange cell-free; DsRed; Discosoma sp. red fluorescent protein; pNPP; p-nitrophenyl palmitate; sfGFP; superfolder green fluorescent protein; Cell-Free protein synthesis; Molecular chaperone; Protein fo
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Keywords: Enzyme activities; Enzyme assays; Phosphatase; Phosphomonoesterase; Michaelis-menton; Sierra nevada; pNP; para-nitrophenol; pNPP; para-nitrophenyl phosphate; PHO; phosphomonoesterase; DOM; dissolved organic matter; SOC; soil organic carbon;
Keywords: ACPA; anti-citrullinated protein antibody; AS; ankylosing spondylitis; AP; alkaline phosphatase; CCP; cyclic citrullinated peptide; EBNA; Epstein-Barr nuclear antigen; h; hour; pNPP; para-nitrophenylphosphate; RA; rheumatoid arthritis; RF; rheumatoid fact
Keywords: Top-down mass spectrometry; Sarcomeres; Post-translational modification; Phosphorylation; AP; alkaline phosphatase; cAMP; cyclic adenosine monophosphate; CK2; casein kinase 2; ECD; electron capture dissociation; ELC; essential light chain; ELISA; enzyme-l
Biochemical and functional analysis of corticotropin releasing factor purified from an aqueous extract of human placenta used as wound healer
Keywords: CHCA; α-cyano-4-hydroxycinnamic acid; EDC; 1-ethyl-3-3-dimethylaminopropyl carbodiimide; ELISA; enzyme linked immunosorbent assay; FBS; fetal bovine serum; IFN-γ; interferon-γ; MES; 2-(N-morpholino)ethanesulfonic acid sodium salt 4-morpholineethanesulf
Keywords: CSP; chemical shift perturbation; PTP; protein tyrosine phosphatase; PTP1B; protein tyrosine phosphatase 1B; VHR; Vaccinia H1-related phosphatase; BB30; benzofuran-based small molecule; pNPP; p-nitrophenylphosphate; SASA; solvent-accessible surface areas;
Keywords: GK; glycerol kinase; LC-MS/MS; liquid chromatography tandem mass spectrometry; G3P; glycerol 3-phosphate; Pi; inorganic phosphate; Tbg; Trypanosoma brucei gambiense; Tbr; Trypanosoma brucei rhodesiense; pNPP; paranitrophenyl phosphate; WHO; World Health O
Keywords: BSA; bovine serum albumin; CK; creatine kinase; DMSO; dimethylsulfoxide; DTNB; 5,5â²-dithio-(2-nitrobenzoic acid); DTT; dithiothreitol; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HBSS; Hank's buffered salt solution without calcium and magnesium; HM
Keywords: ALP; alkaline phosphatase; E2; estradiol; ESRs; nuclear estrogen receptors; ESR1; estrogen receptor 1; ESR2a; estrogen receptor 2a; ESR2b; estrogen receptor 2b; Gen; genistein; GPER; G-protein-coupled estrogen receptor; GPER-like; G-protein-coupled estrog
Keywords: hBM-MSCs; Human bone marrow mesenchymal stromal cells; DCF-DA; 2â²,7â²-Dichlorofluorescein diacetate; ROS; Reactive oxygen species; FAC; Ferric ammonium citrate; SAC; Sodium ammonium citrate; SM; Standard medium; OM; Osteogenic medium; AP; Alkaline phos
Keywords: X-ray crystallography; Histidine acid phosphatase; Histidine phosphatase superfamily; Substrate recognition; HAP; histidine acid phosphatase; LpHAP; Legionella pneumophila histidine acid phosphatase; FtHAP; Francisella tularensis histidine acid phosphatas
Keywords: Sorghum; Polyphenols; Antioxidant capacity; Oxidative stress; Pasta; Durum; B0; maximum binding; CP; control pasta; DNPH; 2,4-dinitrophenylhydrazine; GAE; gallic acid equivalents; H2O2; hydrogen peroxide; NSB; non-specific binding; pNpp; p-nitrophenyl pho
Keywords: PVA; polyvinyl alcohol; OPA; oxidized PVA; OPH; OPA hydrolase; pNP; p-nitrophenyl; pNPA; pNP acetate; pNPB; pNP butyrate; pNPC; pNP caprylate; pNPL; pNP laurate; pNPP; pNP palmitate; DMSO; dimethyl sulfoxide; PEG; polyethylene glycol; DTT; dithiothreitol;
Keywords: PTP; protein tyrosine phosphatase; RCC; redox cycling compounds; ROS; reactive oxygen species; HTS; high-throughput screen; PDB; protein data bank; pNPP; para-nitrophenyl phosphate; VS; virtual screening; DTT; dithiothreitol; pTyr; phosphotyrosine; Protei
Keywords: ALP; alkaline phosphatase; BCA; bicinchoninic acid; O-CMC; O-carboxymethyl chitosan; CaCl2; calcium chloride; CaSO4; calcium sulfate; CaSO4·1/2H2O; calcium sulfate hemihydrate; CFU; colony forming units; DAPI; 4â²,6-diamidino-2-phenylindole; DD; degree
Keywords: AD; Alzheimer's disease; ALS; amyotrophic lateral sclerosis; ANOVA; Analysis of variance; Aβ; amyloid β; bHLH; basic Helix-Loop-Helix; C4; complement C4; CLEAR; coordinated lysosomal expression and regulation; Cyba; cytochrome b-245 light chain; DW; d
Keywords: AD; Alzheimer's disease; ALS; amyotrophic lateral sclerosis; EDTA; ethylenediaminetetraacetic acid; FTLD; frontotemporal lobar degeneration; GFAP; glial fibrillary acidic protein; HE; hematoxylin and eosin; HPRT; hypoxanthine phosphoribosyltransferase;
Structural requirement of the hydrophobic region of the Bordetella pertussis CyaA-hemolysin for functional association with CyaC-acyltransferase in toxin acylation
Keywords: CyaA-RTX; CyaC-acyltransferase; Electrostatic potentials; Hydrophobic region; Palmitoylation; Protein association; AR; acylation region; CyaA; adenylate cyclase-hemolysin toxin; IMAC; immobilized-metal ion affinity chromatography; HP; hydrophobic region;
Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and β-enolases
Keywords: AP; alkaline phosphatase; BSA; bovine serum albumin; ELISA; enzyme-linked immunosorbent assay; ESI; electrospray injection; HRP; horse radish peroxidase; IgG; immunoglobulin G; LC; liquid chromatography; MeOH; methanol; MS; mass spectrometry; OPD; ortho-p
Therapeutic effects of the allosteric protein tyrosine phosphatase 1B inhibitor KY-226 on experimental diabetes and obesity via enhancements in insulin and leptin signaling in mice
Keywords: PTP1B inhibitor; Diabetes; Obesity; Allosteric inhibitor; db/db mouse; EDTA; ethylenediaminetetraacetic acid; FBS; fetal bovine serum; GLUT4; glucose transporter type 4; GPDH; glycerol-3-phosphate dehydrogenase; HbA1c; hemoglobin A1c; HFD; high-fat diet;
Characterization of Caco-2 cells stably expressing the protein-based zinc probe eCalwy-5 as a model system for investigating intestinal zinc transport
Keywords: ANOVA; analysis of variance; ALP; alkaline phosphatase; Calwy; CFP-Atox1-linker-WD4-YFP; DAPI; 4â²,6-diamidin-2-phenylindole; DMEM; Dulbecco's Modified Eagle's Medium; FAAS; flame atomic absorption spectrometry; FCS; fetal calf serum; FLIM; fluoresce
Microcystins: Synthesis and structure-activity relationship studies toward PP1 and PP2A
Keywords: Adda; (2S,3S,8S,9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid; Amba; (2S,3S)-3-amino-2-methylbutanoic acid; Boc; tert-butoxycarbonyl; BOP; benzotriazol-1-yloxy-tris-dimethylaminophosphoniumhexafluoro phosphate; Cbz; carboxybenzyl; D
Inhibition of protein tyrosine phosphatase 1B by flavonoids: A structure - activity relationship study
Keywords: Diabetes mellitus; PTP1B inhibition; Flavonoids; Enzymatic inhibition type; DM; Diabetes mellitus; GLUT4; glucose transporter 4; IR; insulin receptor; IRS; insulin receptor substrates; Km; Michaelis constant; PKB; protein kinase B; pNPP; p-nitrophenyl pho
Substituent effect of N-benzylated gramine derivatives that prevent the PP2A inhibition and dissipate the neuronal Ca2+ overload, as a multitarget strategy for the treatment of Alzheimer's disease
Keywords: ACh; acetylcholine; AD; Alzheimer's disease; Aβ; amyloid β peptide; [Ca2+]c; cytosolic Ca2+; CALHM1; Ca2+ homeostasis modulator-1 channel; FBS; fetal bovine serum; Mem; memantine; NCLX; mitochondrial Na±/Ca2+ exchanger; NFT; neurofibrillary tangles;
Charting novel allergens from date palm pollen (Phoenix sylvestris) using homology driven proteomics
Keywords: MALDI-TOF; Matrix-assisted Laser Desorption Ionisation Time of Flight; IgE; Immunoglobulin E; SPT; skin prick test; pNPP; p-nitrophenyl phosphate; SNP; single nucleotide polymorphism; Date palm; De novo sequencing; Pollen; Allergen; Cross-reactivity; Prot
Structural and functional characterization of a cold adapted TPM-domain with ATPase/ADPase activity
Keywords: pNPP; p-Nitrophenylphosphate; pSer; O-phospho-l-serine; AMP-PCP; β,γ-methyleneadenosine 5â²-triphosphate; SLS; static light scattering; DLS; dynamic light scattering; CD; circular dichroism; NTPDase; nucleoside triphosphate diphosphohydrolase; LCR; low
Reduced levels of cytosolic DNA sensor AIM2 are associated with impaired cytokine responses in healthy elderly
Keywords: TLRs; Toll-like receptors; CLRs; C-type lectin receptors; NLRs; NOD-like receptors; RLRs; retinoic-acid-inducible gene-I like receptors; AIM2; absent in melanoma 2; WBA; whole blood assay; PAMPs; pathogen-associated molecular patterns; PRRs; pattern recog
Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library
Keywords: AP; alkaline phosphatase; CAD HDL; HDL isolated from CAD patients; ctrl HDL; HDL isolated from healthy individuals; rHDL; reconstituted HDL; EFI; europium fluorescence intensifier; IPTG; isopropyl β-D-1-thiogalactopyranoside; PC; phosphatidyl choline; pN
Lipases from the genus Rhizopus: Characteristics, expression, protein engineering and application
Keywords: ROL; R. oryzae lipase; RCL; R. chinensis lipase; RSL; R. stolonifer lipase; RML; Rhizomucor miehei lipase; mROL; the mature R. oryzae lipase; ProROL; the R. oryzae prolipase; r28ROL; the mature R. oryzae lipase containing 28 amino acids of the prosequence
The promiscuous phosphomonoestearase activity of Archaeoglobus fulgidus CopA, a thermophilic Cu+ transport ATPase
Keywords: Af-CopA; Cu(I)-transport ATPase from Archaeglobus fulgidus; DDM; n-dodecyl β-D-maltoside; DTT; Dithiothreitol; HAD; Haloacid dehalogenase; MOPS; 3-(N-morpholino)propanesulfonic acid; PC; Phosphatidylcholine; PE; Phosphatidylethanolamine; Pi; Inorganic ph
Comparison of antibody assays for detection of autoantibodies to Ro 52, Ro 60 and La associated with primary Sjögren's syndrome
Keywords: ANA; anti-nuclear antibodies; AP; alkaline phosphatase; AUC; area under the curve; ELISA; enzyme-linked immunosorbent assay; pNPP; p-nitrophenylphosphate; pSS; primary Sjögren's syndrome; RF; rheumatoid factor; RNP; ribonucleoprotein; RT; room temperatur
Reversible oxidation controls the activity and oligomeric state of the mammalian phosphoglycolate phosphatase AUM
Keywords: CD; circular dichroism; DTT; dithiothreitol; EGF; epidermal growth factor; G3P; glycerol-3-phosphate; HAD; haloacid dehalogenase; IAA; iodoacetic acid; IAP; iodoacetyl polyethylene glycol; LDH; lactate dehydrogenase; MALS; multi angle light scattering; PD
Naringin attenuates the cytotoxicity of hepatotoxin microcystin-LR by the curious mechanisms to OATP1B1- and OATP1B3-expressing cells
Keywords: FCS; fetal calf serum; MC-LR; microcystin-LR; IC50; half maximal inhibitory concentration; Km; Michaelis constant; MEM; minimum essential medium; MTT; 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OATP; organic anion transporting polypept
Osteoblast differentiation profiles define sex specific gene expression patterns in craniosynostosis
Keywords: corOB; primary calvarial osteoblast cell lines from individuals with coronal synostosis; corOB-F; females; corOB-M; males; GO; gene ontology; metOB; primary calvarial osteoblast cell lines from individuals with metopic synostosis; metOB-F; females; metOB-
Na,K-ATPase structure/function relationships probed by the denaturant urea
Keywords: Na,K-ATPase; Na+- and K+-activated ATPase; E1; the protein conformation of Na,K-ATPase predominant in Na+-containing media; E2; the protein conformation of Na,K-ATPase predominant in K+-containing media; K-pNPPase; K+-p-nitrophenylphosphatase; K0.5; denat
Is RK-682 a promiscuous enzyme inhibitor? Synthesis and in vitro evaluation of protein tyrosine phosphatase inhibition of racemic RK-682 and analogues
Keywords: Tyrosine phosphatases inhibitors; Natural 3-acyl-5-hydroxymethyltetronic acid; RK-682; Meldrum's acid derivatives; Enzyme inhibition; Promiscuous enzyme inhibition; PTP-1B; protein tyrosine phosphatase 1B; LMW-PTP; low molecular weight protein tyrosine ph
Identification of a novel Ser/Thr protein phosphatase Ppq1 as a negative regulator of mating MAP kinase pathway in Saccharomyces cerevisiae
Keywords: MAPK; mitogen-activated protein kinase; MAPKK; mitogen-activated protein kinase kinase; MAPKKK; mitogen-activated protein kinase kinase kinase; PP1; Phosphoprotein phosphatase 1; yEGFP; yeast enhanced green fluorescent protein; pNPP; p-nitrophenyl phospha
Effects of C-glycosylation on anti-diabetic, anti-Alzheimer's disease and anti-inflammatory potential of apigenin
Keywords: ACh; acetylthiocholine iodide; AChE; acetylcholinesterase; AGEs; advanced glycation endproducts; AR; aldose reductase; ARI; aldose reductase inhibitor; AMT; 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine hydrochloride; AD; Alzheimer's disease; Aβ; amyloi
Crystal structures of Ophiostoma piceae sterol esterase: Structural insights into activation mechanism and product release
Keywords: pNPB; p-nitrophenylbutyrate; pNPL; p-nitrophenyl laureate; pNPP; p-nitrophenylpalmitate; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; OPE; Ophiostoma piceae esterase; PEG; polyethylene glycol; Fungal lipase/esterase; Activation mechan
The aryl hydrocarbon receptor suppresses osteoblast proliferation and differentiation through the activation of the ERK signaling pathway
Keywords: Ahr; aryl hydrocarbon receptor; AIP; Ahr-interacting protein; ALP; alkaline phosphatase; ARNT; aryl hydrocarbon receptor nuclear translocator; BMD; bone mineral density; CFA; complete Freund's adjuvant; CH-223191; 2-methyl-2H-pyrazole-3-carboxylic acid; C
Emodin suppresses Wnt signaling in human colorectal cancer cells SW480 and SW620
Keywords: CRC; colorectal cancer; APC; adenomatosis polyposis coli; TCF/LEF; T-cell factor / lymphoid enhancer factor; DMSO; dimethyl sulfoxide; PBS; phosphate-buffered saline; DMEM; Dulbecco׳s Modified Eagle Medium; DCFH-DA; 2â²,7â²-dichlorfluorescein-diacetate
The second-sphere residue T263 is important for the function and catalytic activity of PTP1B via interaction with the WPD-loop
Keywords: PTP; protein tyrosine phosphatase; pNPP; p-nitrophenyl phosphate; MUP; 4-methylumbelliferyl phosphate; DiFMUP; 6,8-difluoro-4-methylumbelliferyl phosphate; OPLT; o-phospho-l-tyrosine; HRP; horseradish peroxidase; IPTG; isopropyl-thio-β-d-galactopyranosid
A study of substrate specificity for a CTD phosphatase, SCP1, by proteomic screening of binding partners
Keywords: RNAPII; RNA polymerase II; CTD; C-terminal domain; pNPP; para-nitrophenyl phosphate; SCP; small CTD phosphatase; CTD-SPL; CTD small phosphatase like protein; TIP120A; TATA-binding protein interacting protein 120; CdcA3; Cell division cycle-associated prot
Determinants of the tumor suppressor INPP4B protein and lipid phosphatase activities
Keywords: PI3K; by phosphatidyl 3-kinase; PI4K; phosphatidyl 4-kinase; PI5K; phosphatidyl 5-kinase; INPP4B; inositol polyphosphate 4-phosphatase type II; PTP; protein tyrosine phosphatase; pNPP; para-nitrophenyl phosphate; DifMUP; 6,8-difluoro-4-methylumbelliferyl
Dual inhibition of DNA polymerase PolC and protein tyrosine phosphatase CpsB uncovers a novel antibiotic target
Keywords: PHP; polymerase and histidinol phosphatase; Hpp; histidinol phosphate phosphatase; FQE; fascioquinol E; pNP-TMP; 5â²-p-nitrophenyl ester of thymidine-5â²-monophosphate; pNPP; p-nitrophenyl phosphate; PTP; protein tyrosine phosphatase; Bacteria; Antibiot
Structurally conserved and functionally divergent yeast Ssu72 phosphatases
Keywords: RNAPII; RNA polymerase II; GST; glutathione S-transferase; pNPP; paranitrophenylphosphate; Ser2, Ser5; serine 2 and 5, respectively, from CTD repeats; OD600; optical density at 600 nanometers; CTD; carboxy-terminal domain (CTD) of the major subunit of RNA
Trypanosoma rangeli: An alkaline ecto-phosphatase activity is involved with survival and growth of the parasite
Keywords: β-GP; β-glycerophosphate; pNPP; p-nitrophenyl phosphate; PI-PLC; phosphatidylinositol-phospholipase C; GPI; glycosylphosphatidyl inositol; LIT; liver infusion tryptose medium; FBS; fetal bovine serum; Trypanosoma rangeli; Alkaline ecto-phosphatase; Inor
Contribution of the two domains of E. coli 5â²-nucleotidase to substrate specificity and catalysis
Keywords: 5NT; E. coli 5â²-nucleotidase; AMPCP; α,β-methylene-ADP; NDomN; terminal domain with residues 26-362 and a C-terminal hexahistidine tag; CDom; C-terminal domain with residues 363-550 and a C-terminal hexahistidine tag; pNPP; para-nitrophenyl phosph
Reversal of dexamethasone induced insulin resistance in 3T3L1 adipocytes by 3β-taraxerol of Mangifera indica
Keywords: DEX; dexamethasone; DMEM; Dulbecco's modified Eagle's medium; ELISA; enzyme linked immunosorbent assay; FCS; fetal calf serum; GLUT 4; glucose transporter 4; GS; glycogen synthase; GSK3β; glycogen synthase kinase beta; IR β; insulin receptor beta; IRS1;
A novel arsenate reductase from the bacterium Thermus thermophilus HB27: Its role in arsenic detoxification
Keywords: ars; (arsenic resistance system); aox; (arsenite oxidation system); arr; (arsenate respiratory reduction); TtarsC; (gene ID: 2776273); TM; (Thermus medium); Trx; (thioredoxin); Tr; (thioredoxin reductase); pNPP; (p-nitrophenyl phosphate); MIC; (minimal in