Keywords: Lectins; Molecular farming; Monoclonal antibodies; Plant secondary metabolites; Therapeutic anti-cancer vaccines; APIs; active pharmaceutical ingredients; ADCC; antibody-dependent cellular cytotoxicity; ADCs; antibody-drug conjugates; ATPS; aqueous two-
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Keywords: Breeding methods; Chrysanthemum morifolium; CRISPR/Cas9; Genome editing; Ornamental plants; Transgenic technologies; 4CL; 4-coumarate:coenzyme A ligase; C3H; coumarate 3-hydroxylase; C4H; cinnamate 4-hydroxylase; CCD4; carotenoid cleavage dioxygenase 4; C
Keywords: Cisgenesis; Genome editing; Next generation sequencing; T-DNA; Transgenesis;
Keywords: Bt; Bacillus thuringiensis; CRISPR/Cas9; Clustered Regularly Interspaced Short Palindromic Repeat; DSB; Double stranded breaks; GM; Genetically modified; gRNA; Guide RNA; GURTs; Gene Use Restriction Technologies; HR; Homologous recombination; NHEJ; Non-ho
Keywords: Fluorescence-activated cell sorting; Multilabeled fluorescence marker; DNA nanostructure; Cell sorting; FACS; fluorescence activated cell sorting; PMT; photomultiplier tube; PE/Cy5; phycoerythrin and Cy5; Y-DNA; Y-shaped DNA nanostructure; T-DNA; tree-sha
Keywords: CRISPR; clustered regularly interspaced short palindromic repeats; DSB; double stranded break; EMS; ethyl methane sulfonate; EPA; eicosapentaenoic acid; GMO; genetically modified organism; MNNG; methylnitronitrosoguanidine; TAG; triacylglycerol; TALEs; tr
Keywords: ABA; abscisic acid; ABI1; abscisic acid insensitive 1; AIP1; AKT1 interacting protein phosphatase 1; AKT1; Arabidopsis K+ transporter 1; AtCHX7; cation-proton exchanger 7; AtKC1; Arabidopsis potassium channel α-subunit; AtNHX3; sodium/proton (Na+/H+) ant
Keywords: AMP; antimicrobial peptides; AMY; amylase; HOR3-1; hordein D; GFP; green fluorescent protein; GM; genetically modified; GP; Golden Promise; GUS; β-glucuronidase; PMF; plant molecular farming; T-DNA; transfer DNA; Barley; Transgenesis; Stress tolerance; P
T-DNA associated reciprocal translocation reveals differential survival of male and female gametes
Keywords: Translocation; Insertion line; T-DNA; Gene dosage; Unbalanced chromosome; CCCH zinc finger; dHET; Double heterozygous mutant of AtC3H6 and AtC3H68; T-DNA; Transfer DNA; Col; Arabidpsis thaliana Columbia ecotype; PCR; Polymerase chain reaction; RT-PCR; Rev
Phenotypic characterization of an Arabidopsis T-DNA insertion line SALK_063500
Keywords: Arabidopsis; T-DNA; SALK_063500; Silique; Pollen; Phenotype; AT1G05290;
Functional deficiency of phytochrome B improves salt tolerance in rice
Keywords: phyB; phytochrome B; CBF; C repeat binding factor; DREB; dehydration-responsive element binding protein; PIF; phytochrome-interacting factor; PIL; phytochrome-interacting factor-like; HKT/HAK; high-affinity K+ transporter; MYB; myeloblastosis; bZIP; basic
Application and development of genome editing technologies to the Solanaceae plants
Keywords: Genome editing; Nuclease; Targeted mutagenesis; AGL; agamous-like; AID; activation-induced cytidine deaminase; ALOG; Arabidopsis LSH1 and Oryza G1; ANT; anthocyanin mutant; ARF; auxin-response factor; Aux; auxin-induced; BAK; brassinosteroid kinase; BOP;
Three-dimensional Tri-SNSs-layered electrodeposited reduced graphene oxide for ECL biosensing of DNA
Keywords: 3D-ERGO; Tri-SNSs; Ru2-DNA; T-DNA; ECL; Biosensor;
A potential efflux boron transporter gene GsBOR2, positively regulates Arabidopsis bicarbonate tolerance
Keywords: A. thaliana; Arabidopsis thaliana; BOR; high boron requiring; G. max; Glycine max; G. soja; Glycine soja; LiAc; lithium acetate; M. truncatula; Medicago truncatula; MDA; malondialdehyde; MES; 2- (N-Morpholino) ethanesulfonic acid; MS medium; Murashige and
A stress associated NAC transcription factor MpSNAC67 from banana (Musa x paradisiaca) is involved in regulation of chlorophyll catabolic pathway
Keywords: Musa; Transgenic; Banana; SNAC67; Gene expression; Chlorophyll degradation; VND; vascular-related NAC transcription factors; GUS; β-D-Glucuronidase; SD; Synthetic Defined; NBT/BCIP; nitro-blue tetrazolium/5-bromo-4-chloro-3â²-indolyphosphate; T-DNA; tra
Single molecule Raman spectroscopic assay to detect transgene from GM plants
Keywords: Genetically modified plants (GMOs); Surface-enhanced Raman spectroscopy (SERS); Biosensors; Transgene detection; CaMV-35S; T-DNA; NPTII gene;
Highly efficient transformation system for Malassezia furfur and Malassezia pachydermatis using Agrobacterium tumefaciens-mediated transformation
Keywords: Malassezia spp.; Transformation; T-DNA; Agrobacterium; Hygromycin; GFP;
Data for characterization of SALK_084889, a T-DNA insertion line of Arabidopsis thaliana
Keywords: Arabidopsis; DREB2A; LOX1; SALK_084889; T-DNA;
BIBAC-GW-based vectors for generating reporter lines for site-specific genome editing in planta
Keywords: BIBAC; Binary vector based on Bacterial artificial chromosomes; ChIP; Chromatin ImmunoPrecipitation; CRISPR; Clustered Regularly-Interspaced Short Palindromic Repeat; GW; Gateway; NLS; nuclear localization signal; Nos; Nopaline Synthase; ODM; Oligonucleot
A 64-bp sequence containing the GAAGA motif is essential for CaMV-35S promoter methylation in gentian
Keywords: CaMV; cauliflower mosaic virus; sGFP; synthetic green fluorescent protein; T-DNA; transfer DNA; DNA methylation; Gentian; 35S promoter; Transgene silencing; De novo methylation;
Suppression of PCD-related genes affects salt tolerance in Arabidopsis
Keywords: Knockdown; VIGS; Knockout; T-DNA; sqRT-PCR
Peroxisome biogenesis, protein targeting mechanisms and PEX gene functions in plants
Keywords: AAA; ATPases Associated with various cellular Activities; APEM; Aberrant PEroxisome Morphology; EosFP; Eos Fluorescent protein; ERAD; ER Associated Decay; GET; Guided Entry of TA proteins; pER; peroxisomal ER subdomain; PEX; abbreviation for Peroxin, a pr
Production of dammarane-type sapogenins in rice by expressing the dammarenediol-II synthase gene from Panax ginseng C.A. Mey
Keywords: DS; Dammarenediol-II synthase; T-DNA; Transfer-DNA; HPLC; High performance liquid chromatography; PPD; 20 (S)-Protopanaxadiol; PPT; 20 (S)-Protopanaxatriol; DAD; Dammarenediol-II; ESI; Electrospray ionization; EIC; Extracted ion chromatogram; PSY; Phyto
Increasing the stearate content in seed oil of Brassica juncea by heterologous expression of MlFatB affects lipid content and germination frequency of transgenic seeds
Keywords: Brassica juncea (Indian mustard); Erucic acid; FatB thioesterase; Fatty acid biosynthesis; Metabolic engineering; Seed lipid; Stearic acid; ACP; Acyl carrier protein; Fat; Fatty acyl-ACP thioesterase; BSA; Bovine serum albumin; BSE; Bovine spongiforme enc
Manipulation of epigenetic factors and the DNA repair machinery for improving the frequency of plant transformation
Keywords: C-NHEJ; canonical NHEJ; DSBs; DNA double-strand breaks; gDNA; genomic DNA; GT; gene targeting; HR; homologous recombination; NHEJ; non-homologous end joining; PTGS; posttranscriptional gene silencing; RNAi; RNA interference; rat; resistant to Agrobacteriu
A rice-based soluble form of a murine TNF-specific llama variable domain of heavy-chain antibody suppresses collagen-induced arthritis in mice
Keywords: Ab; antibody; CIA; collagen-induced arthritis; CII; bovine type II collagen; CTB; cholera toxin B subunit; ELISA; enzyme-linked immunosorbent assay; GST; glutathione S-transferase; mTNF; mouse tumor necrosis factor alpha; PBS; phosphate buffered saline; R
The Arabidopsis thaliana At4g13040 gene, a unique member of the AP2/EREBP family, is a positive regulator for salicylic acid accumulation and basal defense against bacterial pathogens
Keywords: AP2; Apetala 2; APD1; Apetala 2 family protein involved in SA mediated disease defense; Avr-Pst; avirulent strain of Pseudomonas syringae pv. tomato DC3000; DAPI; 4â²,6-diamidino-2-phenylindole; dpi; days post inoculation; DREB; dehydration response elem
Spectrum of T-DNA integrations for insertional mutagenesis of Histoplasma capsulatum
Keywords: Agrobacterium; Histoplasma; Insertional mutagenesis; T-DNA; TAIL-PCR
Heterogeneous electrochemiluminescence spectrometry of Ru(bpy)32+ for determination of trace DNA and its application in measurement of gene expression level
Keywords: B-p-DNA; biotinylated probe DNA; bpy; 2,2â²-bipyridyl; dcbpy; 2,2â²-bipyridine-4,4â²-dicarboxylic acid; DEPC; diethypyrocarbonate; DMF; Nâ²,N-dimethylformamide; DNase; deoxyribonuclease; dNTP; deoxy-ribonucleoside triphosphate; ECL; electrochemilumine
Interpreting the biological relevance of bioinformatic analyses with T-DNA sequence for protein allergenicity
Keywords: EST; expressed sequence tag; FARRP; Food Allergy Research and Resource Program; ORF; open reading frame; T-DNA; transfer DNA; UTR; untranslated region; Transgenic; Crops; T-DNA; Allergenicity; Bioinformatics; Sequence; Biotechnology; Protein; Genome; Prot
Efficient insertional mutagenesis system for the dimorphic pathogenic fungus Sporothrix schenckii using Agrobacterium tumefaciens
Keywords: ATMT; Agrobacterium tumefaciens-mediated transformation; T-DNA; transferred DNA; AS; acetosyringone; IM; induction medium; SM; selection medium; hph; phosphotransferase gene; TAIL-PCR; thermal asymmetric interlaced PCR; LB; left border; RB; right border;
NKS1, Na+- and K+-sensitive 1, regulates ion homeostasis in an SOS-independent pathway in Arabidopsis
Keywords: T-DNA; transfer DNA; SOS; salt overly sensitive; UTR; untranslated region; ER; endoplasmic reticulum; GFP; green fluorescent protein; RFP; red fluorescent protein; GUS; beta-glucuronidase; Arabidopsis thaliana; Cruciferae; Salt stress; NaCl stress; Ion ho
Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
Keywords: Arabidopsis; Carriers; Chloroplasts; Correlation analysis; Iron; Mitochondria; Mitoferrin; AIS; Arabidopsis growth medium; Col; Columbia; DW; dry weight; EDTA; ethylenediaminetetracetic acid; KO; knock out; EDDHA; ethylenediamine-N,Nâ²-bis(2-hydroxypheny
Induction of chromosomal inversion by integration of T-DNA in the rice genome
Keywords: Agrobacterium tumefaciens-mediated transformation (ATMT); T-DNA; chromosomal inversion
Overexpression of ACL1 (abaxially curled leaf 1) Increased Bulliform Cells and Induced Abaxial Curling of Leaf Blades in Rice
Keywords: Cell expansion; leaf/vegetative development; rice; ACL1; ACL2; LRI; T-DNA; bulliform cells
The aux1 gene of the Ri plasmid is sufficient to confer auxin autotrophy in tobacco BY-2 cells
Keywords: Agrobacterium rhizogenes; Auxin-autonomous cell lines; Ri plasmid; T-DNA; Tobacco BY-2 cellsBY-2, Bright Yellow-2; CaMV, cauliflower mosaic virus; Cef, sodium cefotaxime; IAA, indole-3-acetic acid; IAM, indole-3-acetamide; Km, kanamycin sulfate; LS, Linsm
Size reversion of a truncated DNAβ associated with Tobacco curly shoot virus
Keywords: Tobacco curly shoot virus; βC1 gene; DNAβ; Size reversion; Recombinant; T-DNA;
The roles of the atDjA2 and atDjA3 molecular chaperone proteins in improving thermotolerance of Arabidopsis thaliana seedlings
Keywords: HS, heat shock; Hsps, heat shock proteins; HSF, heat shock transcription factor; WT, wild typeArabidopsis; Gene expression; J-domain protein; Molecular chaperone; T-DNA; Thermotolerance
Purification and characterization of an intracellular peroxidase from genetically transformed roots of red beet (Beta vulgaris L.)
Keywords: Agrobacterium rhizogenes; Hairy root; T-DNA; Transformation; Peroxidase; HRP; Purification; Characterization; Isozymes
Repair competence assay in studies of the influence of environmental exposure to c-PAHs on individual susceptibility to induction of DNA damage
Keywords: c-PAHs; carcinogenic polycyclic aromatic hydrocarbons; B[a]A; benzo[a]anthracene; CHRY; chrysene; B[b]F; benzo[b]fluoranthene; B[k]F; benzo[k]fluoranthene; B[a]P; benzo[a]pyrene; DB[a,h]A; dibenzo[a,h]anthracene; B[g,h,l]P; benzo[g,h,l]perylene; I[c,d]P;
Effect of chromatin upon Agrobacterium T-DNA integration and transgene expression
Keywords: Agrobacterium; Plant genetic engineering; T-DNA; Transgene; Chromatin; Methylation
Analysis of T-DNA insertion site distribution patterns in Arabidopsis thaliana reveals special features of genes without insertions
Keywords: T-DNA; Integration; Insertional mutagenesis; Arabidopsis thaliana; Restriction sites; Gene expression
Transformation of Coniothyrium minitans, a parasite of Sclerotinia sclerotiorum, with Agrobacterium tumefaciens
Keywords: Coniothyrium minitans; Agribacterium tumefaciens; Sclerotinia sclerotiorum; Biological control; T-DNA insertion; ATMT of filamentous fungi; T-DNA; Sporulation; Pathogenicity;
Unique genetic compartmentalization of the SUF system in cryptophytes and characterization of a SufD mutant in Arabidopsis thaliana
Keywords: ABC; ATP binding cassette; EST; expressed sequence tag; Fe-S; iron-sulfur; GFP; green fluorescent protein; GST; glutathione S-transferase; ISC; iron-sulfur cluster assembly machinery; NAP; non-intrinsic ABC protein; NIF; nitrogen fixation; SUF; mobi
High efficiency of genetic transformation and regeneration of Artemisia annua L. via Agrobacterium tumefaciens-mediated procedure
Keywords: GFP; green fluorescent protein; NAA; α-naphthaleneacetic acid; 6-BA; 6-benzylaminopurine; As; acetosyringone; Kan; kanamycin; Cefo; cefotaxime; PCR; polymerase chain reaction; bp; base pairs; T-DNA; transfer-DNA; Artemisia annua L.; Agrobacterium tumefac
Influence of environmental exposure to PAHs on the susceptibility of lymphocytes to DNA-damage induction and on their repair capacity
Keywords: B[a]A; benz[a]anthracene; B[a]P; benzo[a]pyrene; B[b]F; benzo[b]fluoranthene; B[g,h,l]P; benzo[g,h,l]perylene; B[k]F; benzo[k]fluoranthene; CHRY; chrysene; c-PAHs carcinogenic polycyclic aromatic hydrocarbons; DB[a,h]A; dibenzo[a,h]anthracene; I[c,d]P; in
Functional analysis of GUS expression patterns and T-DNA integration characteristics in rice enhancer trap lines
Keywords: Enhancer trap; GAL4/VP16-UAS-GUS; PCR walking; Promoter; Rice; T-DNA;
Characterization of the rolB promoter on mikimopine-type pRi1724 T-DNA
Keywords: GUS; β-glucuronidase; IAA; indoleacetic acid; NtBBF1; Nicotiana tabacum rolB domain B factor 1; NAA; naphthaleneacetic acid; ORF; open reading frame; rolB; rooting locus B; T-DNA; transferred-DNA; Agrobacterium rhizogenes; Auxin; cis-element; GUS; Ri pla
Transcriptional analysis and functional genomics in wheat
Keywords: Wheat; Functional genomics; Microarray; Expression; AFLP; amplified fragment-length polymorphism; dpa; days post-anthesis; EMS; ethylmethane sulphonate; EST; expressed sequence tags; FACS; fluorescence activated cell sorting; GEM; gene expression microarr