mRNA and 18S-RNA coapplication-reverse transcription for quantitative gene expression analysis Fulltext Access 8 Pages 2005
Conserved protein TTHA1554 from Thermus thermophilus HB8 binds to glutamine synthetase and cystathionine β-lyase Fulltext Access 8 Pages 2005
Mutagenesis of Ala290, which modulates substrate subsite affinity at the catalytic interface of dimeric ThMA Fulltext Access 8 Pages 2005
A colorimetric determination for glycosidic and bile salt-based detergents: applications in membrane protein research Fulltext Access 8 Pages 2005
Applications of capillary isoelectric focusing with liquid-core waveguide laser-induced fluorescence whole-column imaging detection Fulltext Access 8 Pages 2005
A comparison of different strategies for the construction of amperometric enzyme biosensors using gold nanoparticle-modified electrodes Fulltext Access 8 Pages 2005
Simplex optimization of the variables affecting the micelle-stabilized room temperature phosphorescence of 6-methoxy-2-naphthylacetic acid and its kinetic determination in human urine Fulltext Access 8 Pages 2005
Quantitative analysis of sphingoid base-1-phosphates as bisacetylated derivatives by liquid chromatography-tandem mass spectrometry Fulltext Access 8 Pages 2005
Comparison of full-length versus ligand binding domain constructs in cell-free and cell-based peroxisome proliferator-activated receptor alpha assays Fulltext Access 8 Pages 2005
Radiochromatographic assay of N-acyl-phosphatidylethanolamine-specific phospholipase D activity Fulltext Access 8 Pages 2005
Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries Fulltext Access 8 Pages 2005
Electrochemical study of the interaction between cytochrome c and DNA at a modified gold electrode Fulltext Access 8 Pages 2005
The improvement of sensitivity in lead and cadmium determinations using flame atomic absorption spectrometry Fulltext Access 8 Pages 2005
Deglycosylation of glucoamylase from Aspergillus niger: Effects on structure, activity and stability Fulltext Access 8 Pages 2005
Isolation and characterization of subcellular protein fractions from mouse heart Fulltext Access 8 Pages 2005
Microfluidic systems and proteomics: Applications of the electrocapture technology to protein and peptide analysis Fulltext Access 8 Pages 2005
Determination of denaturated proteins and biotoxins by on-line size-exclusion chromatography-digestion-liquid chromatography-electrospray mass spectrometry Fulltext Access 8 Pages 2005
Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests Fulltext Access 8 Pages 2005
Automated expression and solubility screening of His-tagged proteins in 96-well format Fulltext Access 8 Pages 2005
DigiTag assay for multiplex single nucleotide polymorphism typing with high success rate Fulltext Access 8 Pages 2005
A sensitive immunoassay based on electropolymerized films by capacitance measurements for direct detection of immunospecies Fulltext Access 8 Pages 2005
Functional magnetic resonance imaging based on SEEP contrast: response function and anatomical specificity Fulltext Access 8 Pages 2005
Implementation of a rapid inversion-prepared dual-contrast gradient echo sequence for quantitative dynamic contrast-enhanced magnetic resonance imaging of the human prostate Fulltext Access 8 Pages 2005
Expression and spectroscopic characterization of a large fragment of the μ-opioid receptor Fulltext Access 8 Pages 2005
Effect of genetic variation on the thermal stability of human serum albumin Fulltext Access 8 Pages 2005
Molecular simulation studies of a selenium-containing scFv catalytic antibody that mimics glutathione peroxidase Fulltext Access 8 Pages 2005
The role of tryptophan residues in the autoprocessing of prosubtilisin E Fulltext Access 8 Pages 2005
G148-GA3: A streptococcal virulence module with atypical thermodynamics of folding optimally binds human serum albumin at physiological temperatures Fulltext Access 8 Pages 2005
A preliminary account of the properties of recombinant human Glyoxylate reductase (GRHPR), LDHA and LDHB with glyoxylate, and their potential roles in its metabolism Fulltext Access 8 Pages 2005
Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization Fulltext Access 8 Pages 2005
Preparation and characterization of a single-chain calcineurin-calmodulin complex Fulltext Access 8 Pages 2005
Understanding molecular mechanisms of proteolysis in Alzheimer's disease: Progress toward therapeutic interventions Fulltext Access 8 Pages 2005
Measurement of steroid synthesis in zona glomerulosa cells by liquid chromatography-electrospray ionization-mass spectrometry: inhibition by nitric oxide Fulltext Access 8 Pages 2005
Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 Ã ) and biochemical basis Fulltext Access 8 Pages 2005
Comparison of rat and human alkaline phosphatase isoenzymes and isoforms using HPLC and electrophoresis Fulltext Access 8 Pages 2005
Elevated expression temperature in a mesophilic host results in increased secretion of a hyperthermophilic enzyme and decreased cell stress Fulltext Access 8 Pages 2005
Beta2-microglobulin removal by extracorporeal renal replacement therapies Fulltext Access 8 Pages 2005
From chance to frequent encounters: Origins of β2-microglobulin fibrillogenesis Fulltext Access 8 Pages 2005
Cloning and expression of ostrich trypsinogen: an avian trypsin with a highly sensitive autolysis site Fulltext Access 8 Pages 2005
Effect of the polypeptide binding on the thermodynamic stability of the substrate binding domain of the DnaK chaperone Fulltext Access 8 Pages 2005
Calcium-induced conformational changes of the recombinant CBP3 protein from Dictyostelium discoideum Fulltext Access 8 Pages 2005
A common docking site for response regulators on the yeast phosphorelay protein YPD1 Fulltext Access 8 Pages 2005
Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases Fulltext Access 8 Pages 2005
Activity assay of mammalian 2-cys peroxiredoxins using yeast thioredoxin reductase system Fulltext Access 8 Pages 2005
Discrimination between closed and open forms of lipases using electrophoretic techniques Fulltext Access 8 Pages 2005
A fluorescence resonance energy transfer-based probe to monitor nucleosome structure Fulltext Access 8 Pages 2005
Evaluation of alkoxyresorufins as fluorescent substrates for cytochrome P450 BM3 and site-directed mutants Fulltext Access 8 Pages 2005
Rapid simultaneous determination of metabolic clearance of multiple compounds catalyzed in vitro by recombinant human UDP-glucuronosyltransferases Fulltext Access 8 Pages 2005
Intracellular monitoring of superoxide dismutase expression in an Escherichia coli fed-batch cultivation using on-line disruption with at-line surface plasmon resonance detection Fulltext Access 8 Pages 2005
Biochemical and cell-based assays for characterization of BACE-1 inhibitors Fulltext Access 8 Pages 2005
Monitoring the acetohydroxy acid synthase reaction and related carboligations by circular dichroism spectroscopy Fulltext Access 8 Pages 2005
A continuous spectrophotometric assay for human cystathionine beta-synthase Fulltext Access 8 Pages 2005
Conformational impurity of disulfide proteins: Detection, quantification, and properties Fulltext Access 8 Pages 2005
Application of high-performance liquid chromatography-electrospray ionization-mass spectrometry to measure microsomal membrane transport of glucuronides Fulltext Access 8 Pages 2005
Development of a mechanism-based assay for tissue transglutaminase-results of a high-throughput screen and discovery of inhibitors Fulltext Access 8 Pages 2005
Detection of amino acid and peptide phosphate protonation using Raman spectroscopy Fulltext Access 8 Pages 2005
The critical role of methionine 35 in Alzheimer's amyloid β-peptide (1-42)-induced oxidative stress and neurotoxicity Fulltext Access 8 Pages 2005
Quantifying ERK2-protein interactions by fluorescence anisotropy: PEA-15 inhibits ERK2 by blocking the binding of DEJL domains Fulltext Access 8 Pages 2005
Development of specific Rho-kinase inhibitors and their clinical application Fulltext Access 8 Pages 2005
P-Ser-HPr-a link between carbon metabolism and the virulence of some pathogenic bacteria Fulltext Access 8 Pages 2005
Functional studies of protein tyrosine phosphatases with chemical approaches Fulltext Access 8 Pages 2005
A spectrophotometric method for the quantification of an enzyme activity producing 4-substituted phenols: Determination of toluene-4-monooxygenase activity Fulltext Access 8 Pages 2005
Continuous-flow/stopped-flow system using an immunobiosensor for quantification of human serum IgG antibodies to Helicobacter pylori Fulltext Access 8 Pages 2005
Optical zymography for specific detection of urokinase plasminogen activator activity in biological samples Fulltext Access 8 Pages 2005
A novel means of asymmetric linker attachment to DNA molecule by RecA-mediated multistranded DNA formation Fulltext Access 8 Pages 2005
Analytical method for ubiquinone-9 and ubiquinone-10 in rat tissues by liquid chromatography/turbo ion spray tandem mass spectrometry with 1-alkylamine as an additive to the mobile phase Fulltext Access 8 Pages 2005
Tandem mass spectrometric assay of succinylacetone in urine for the diagnosis of hepatorenal tyrosinemia Fulltext Access 8 Pages 2005
Rapid sodium cyanide depletion in cell culture media: Outgassing of hydrogen cyanide at physiological pH Fulltext Access 8 Pages 2005
Alkaline phosphatase vs luciferase as secreted reporter molecules in vivo Fulltext Access 8 Pages 2005
The preparation of HEK293 α1A or HEK293 α1B cell membrane stationary phase and the chromatographic affinity study of ligands of α1 adrenoceptor Fulltext Access 8 Pages 2005
Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe Fulltext Access 8 Pages 2005
Time-resolved Forster resonance energy transfer assays for the binding of nucleotide and protein substrates to p38α protein kinase Fulltext Access 8 Pages 2005
Analysis of hyaluronan content in chondroitin sulfate preparations by using selective enzymatic digestion and electrospray ionization mass spectrometry Fulltext Access 8 Pages 2005
Signaling protein inhibitors via the combinatorial modification of peptide scaffolds Fulltext Access 8 Pages 2005
Immobilization and hybridization of oligonucleotides on maleimido-terminated self-assembled monolayers Fulltext Access 8 Pages 2005
An ultrasensitive fluorescent assay for the in vivo quantification of superoxide radical in organisms Fulltext Access 8 Pages 2005
Synthesis and study of the electrophoretic behavior of mannan conjugates with cyclic peptide analogue of myelin basic protein using lysine-glycine linker Fulltext Access 8 Pages 2005
pH determination by pyranine: Medium-related artifacts and their correction Fulltext Access 8 Pages 2005
Paclitaxel quantification in mouse plasma and tissues containing liposome-entrapped paclitaxel by liquid chromatography-tandem mass spectrometry: application to a pharmacokinetics study Fulltext Access 8 Pages 2005
Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly 13C-labeled cell extracts as internal standards Fulltext Access 8 Pages 2005
New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins Fulltext Access 8 Pages 2005
Real-time polymerase chain reaction-based exponential sample amplification for microarray gene expression profiling Fulltext Access 8 Pages 2005
Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry Fulltext Access 8 Pages 2005
Detection of myofibrillar proteins using a step gradient minigel with an ambiguous interface Fulltext Access 8 Pages 2005