A semi-automated large-scale process for the production of recombinant tagged proteins in the Baculovirus expression system Fulltext Access 11 Pages 2006
Synthesis of chicken major histocompatibility complex class II oligomers using a baculovirus expression system Fulltext Access 11 Pages 2006
Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1 Fulltext Access 11 Pages 2006
Production of a truncated soluble human semicarbazide-sensitive amine oxidase mediated by a GST-fusion protein secreted from HEK293 cells Fulltext Access 11 Pages 2006
Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity Fulltext Access 11 Pages 2006
Bacterial expression of functional, biotinylated peripheral cannabinoid receptor CB2 Fulltext Access 11 Pages 2006
Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells Fulltext Access 11 Pages 2006
Cloning, expression, purification, and properties of a putative plasma membrane hexokinase from Solanum chacoense Fulltext Access 11 Pages 2006
Synthesis and assembly of an adjuvanted Porphyromonas gingivalis fimbrial antigen fusion protein in plants Fulltext Access 11 Pages 2006
Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody Fulltext Access 11 Pages 2006
Expression and purification of recombinant human receptor for advanced glycation endproducts in Escherichia coli Fulltext Access 11 Pages 2006
Purification and characterization of the CK2α′-based holoenzyme, an isozyme of CK2α: A comparative analysis Fulltext Access 11 Pages 2006
Cloning, sequencing, expression, and characterization of protealysin, a novel neutral proteinase from Serratia proteamaculans representing a new group of thermolysin-like proteases with short N-terminal region of precursor Fulltext Access 11 Pages 2006
Directed evolution for improved secretion of cancer–testis antigen NY-ESO-1 from yeast Fulltext Access 11 Pages 2006
Pre-emptive and continuous inhaled NO counteracts the cardiopulmonary consequences of extracorporeal circulation in a pig model Fulltext Access 11 Pages 2006
Catalase protects cardiomyocytes via its inhibition of nitric oxide synthesis Fulltext Access 11 Pages 2006
Nitric oxide-mediated inhibition of DNA synthesis was attenuated in hypertrophied neonatal rat ventricular myocytes Fulltext Access 11 Pages 2006
Effects of repeated in vivo inhalant nitrite exposure on gene expression in mouse liver and lungs Fulltext Access 11 Pages 2006
Poly(ADP-ribose) polymerase activation by reactive nitrogen species-Relevance for the pathogenesis of inflammation Fulltext Access 11 Pages 2006
Cerebrovascular protection by various nitric oxide donors in rats after experimental stroke Fulltext Access 11 Pages 2006
Nitric oxide donor-induced persistent inhibition of cell adhesion protein expression and NFκB activation in endothelial cells Fulltext Access 11 Pages 2006
Mechanisms underlying dysfunction of carotid arteries in genetically hyperlipidemic rabbits Fulltext Access 11 Pages 2006
Differential expression of genes involved in cGMP-dependent nitric oxide signaling in murine embryonic stem (ES) cells and ES cell-derived cardiomyocytes Fulltext Access 11 Pages 2006
Control of BRCA2 Cellular and Clinical Functions by a Nuclear Partner, PALB2 Fulltext Access 11 Pages 2006
The Molecular Basis of Filamin Binding to Integrins and Competition with Talin Fulltext Access 11 Pages 2006
Bridging Conformational Dynamics and Function Using Single-Molecule Spectroscopy Fulltext Access 11 Pages 2006
Lumbricus Erythrocruorin at 3.5 Å Resolution: Architecture of a Megadalton Respiratory Complex Fulltext Access 11 Pages 2006
Crystal Structure of Rab11 in Complex with Rab11 Family Interacting Protein 2 Fulltext Access 11 Pages 2006
Prokaryotic Type II and Type III Pantothenate Kinases: The Same Monomer Fold Creates Dimers with Distinct Catalytic Properties Fulltext Access 11 Pages 2006
A Steric Antagonism of Actin Polymerization by a Salmonella Virulence Protein Fulltext Access 11 Pages 2006
Structural Insight into Interactions between Dihydrolipoamide Dehydrogenase (E3) and E3 Binding Protein of Human Pyruvate Dehydrogenase Complex Fulltext Access 11 Pages 2006
Principles of Protein-DNA Recognition Revealed in the Structural Analysis of Ndt80-MSE DNA Complexes Fulltext Access 11 Pages 2006
Structural Determinants for High-Affinity Binding in a Nedd4 WW3∗ Domain-Comm PY Motif Complex Fulltext Access 11 Pages 2006
Structure of a Leu3-DNA Complex: Recognition of Everted CGG Half-Sites by a Zn2Cys6 Binuclear Cluster Protein Fulltext Access 11 Pages 2006
The Crystal Structure of Carnitine Palmitoyltransferase 2 and Implications for Diabetes Treatment Fulltext Access 11 Pages 2006
The Endosome-Associated Protein Hrs Is Hexameric and Controls Cargo Sorting as a “Master Molecule” Fulltext Access 11 Pages 2006
Genomic Association of the Proteasome Demonstrates Overlapping Gene Regulatory Activity with Transcription Factor Substrates Fulltext Access 11 Pages 2006
Two Structurally Atypical HEAT Domains in the C-Terminal Portion of Human eIF4G Support Binding to eIF4A and Mnk1 Fulltext Access 11 Pages 2006
Structures and Polymorphic Interactions of Two Heptad-Repeat Regions of the SARS Virus S2 Protein Fulltext Access 11 Pages 2006
YfhJ, a Molecular Adaptor in Iron-Sulfur Cluster Formation or a Frataxin-like Protein? Fulltext Access 11 Pages 2006
RNA Kink-Turns as Molecular Elbows: Hydration, Cation Binding, and Large-Scale Dynamics Fulltext Access 11 Pages 2006
High yield synthesis and characterization of phosphorylated recombinant human procathepsin D expressed in mammalian cells Fulltext Access 11 Pages 2006
A C-terminal glycine suppresses production of pleurocidin as a fusion peptide in Escherichia coli Fulltext Access 11 Pages 2006
High yield of biologically active recombinant human amelogenin using the baculovirus expression system Fulltext Access 11 Pages 2006
Expression in yeast and purification of a membrane protein, SERCA1a, using a biotinylated acceptor domain Fulltext Access 11 Pages 2006
Affinity purification, physicochemical and immunological characterization of a galactose-specific lectin from the seeds of Dolichos lablab (Indian lablab beans) Fulltext Access 11 Pages 2006
Expression and purification of His-tagged HPV16 E7 protein active in pRb binding Fulltext Access 11 Pages 2006
A Branched Pathway Governing the Activation of a Developmental Transcription Factor by Regulated Intramembrane Proteolysis Fulltext Access 11 Pages 2006
The Structural Basis of Cooperative Regulation at an Alternate Genetic Switch Fulltext Access 11 Pages 2006
Elongation Arrest by SecM via a Cascade of Ribosomal RNA Rearrangements Fulltext Access 11 Pages 2006
Transcription Impairment and Cell Migration Defects in Elongator-Depleted Cells: Implication for Familial Dysautonomia Fulltext Access 11 Pages 2006
PIASxα Differentially Regulates the Amplitudes of Transcriptional Responses Following Activation of the ERK and p38 MAPK Pathways Fulltext Access 11 Pages 2006
HDAC1 Acetylation Is Linked to Progressive Modulation of Steroid Receptor-Induced Gene Transcription Fulltext Access 11 Pages 2006
Molecular Recognition of Transcriptional Repressor Motifs by the WD Domain of the Groucho/TLE Corepressor Fulltext Access 11 Pages 2006
Cell Cycle and Checkpoint Regulation of Histone H3 K56 Acetylation by Hst3 and Hst4 Fulltext Access 11 Pages 2006
A Basal Promoter Element Recognized by Free RNA Polymerase σ Subunit Determines Promoter Recognition by RNA Polymerase Holoenzyme Fulltext Access 11 Pages 2006
Structural Biology of RNA Polymerase III: Subcomplex C17/25 X-Ray Structure and 11 Subunit Enzyme Model Fulltext Access 11 Pages 2006
Structural Basis for Polypyrimidine Tract Recognition by the Essential Pre-mRNA Splicing Factor U2AF65 Fulltext Access 11 Pages 2006
Growth Factor Signaling Regulates Elongation of RNA Polymerase I Transcription in Mammals via UBF Phosphorylation and r-Chromatin Remodeling Fulltext Access 11 Pages 2006
Essential Role for IκB Kinase β in Remodeling Carma1-Bcl10-Malt1 Complexes upon T Cell Activation Fulltext Access 11 Pages 2006
A Mechanism of Nucleotide Misincorporation during Transcription due to Template-Strand Misalignment Fulltext Access 11 Pages 2006
Two Functional Modes of a Nuclear Receptor-Recruited Arginine Methyltransferase in Transcriptional Activation Fulltext Access 11 Pages 2006
Opposing Polymerase-Deadenylase Activities Regulate Cytoplasmic Polyadenylation Fulltext Access 11 Pages 2006
The Transition between Transcriptional Initiation and Elongation in E. coli Is Highly Variable and Often Rate Limiting Fulltext Access 11 Pages 2006
The Polycomb Protein Ring1B Generates Self Atypical Mixed Ubiquitin Chains Required for Its In Vitro Histone H2A Ligase Activity Fulltext Access 11 Pages 2006
Histidine Phosphorylation of the Potassium Channel KCa3.1 by Nucleoside Diphosphate Kinase B Is Required for Activation of KCa3.1 and CD4 T Cells Fulltext Access 11 Pages 2006
The EF-G-like GTPase Snu114p Regulates Spliceosome Dynamics Mediated by Brr2p, a DExD/H Box ATPase Fulltext Access 11 Pages 2006
Structural Basis for CoREST-Dependent Demethylation of Nucleosomes by the Human LSD1 Histone Demethylase Fulltext Access 11 Pages 2006
Locus Control Region Transcription Plays an Active Role in Long-Range Gene Activation Fulltext Access 11 Pages 2006
Rad17 Phosphorylation Is Required for Claspin Recruitment and Chk1 Activation in Response to Replication Stress Fulltext Access 11 Pages 2006
RNA Polymerase Pausing Regulates Translation Initiation by Providing Additional Time for TRAP-RNA Interaction Fulltext Access 11 Pages 2006
The Molecular Basis of Vitamin D Receptor and β-Catenin Crossregulation Fulltext Access 11 Pages 2006
XIAP Is a Copper Binding Protein Deregulated in Wilson's Disease and Other Copper Toxicosis Disorders Fulltext Access 11 Pages 2006
Single-Cell Observations Reveal Intermediate Transcriptional Silencing States Fulltext Access 11 Pages 2006
Nucleocytosolic Acetyl-Coenzyme A Synthetase Is Required for Histone Acetylation and Global Transcription Fulltext Access 11 Pages 2006
How Initiation Factors Maximize the Accuracy of tRNA Selection in Initiation of Bacterial Protein Synthesis Fulltext Access 11 Pages 2006
Cyanobacterial Circadian Pacemaker: Kai Protein Complex Dynamics in the KaiC Phosphorylation Cycle In Vitro Fulltext Access 11 Pages 2006
Nuclear PtdIns5P as a Transducer of Stress Signaling: An In Vivo Role for PIP4Kbeta Fulltext Access 11 Pages 2006
Selective Di- or Trimethylation of Histone H3 Lysine 76 by Two DOT1 Homologs Is Important for Cell Cycle Regulation in Trypanosoma brucei Fulltext Access 11 Pages 2006
SCFβTrCP-Mediated Degradation of Claspin Regulates Recovery from the DNA Replication Checkpoint Response Fulltext Access 11 Pages 2006
Crystal Structure of Pseudouridine Synthase RluA: Indirect Sequence Readout through Protein-Induced RNA Structure Fulltext Access 11 Pages 2006
Identification of a Rapid Mammalian Deadenylation-Dependent Decay Pathway and Its Inhibition by a Viral RNA Element Fulltext Access 11 Pages 2006
The Checkpoint Clamp Activates Mec1 Kinase during Initiation of the DNA Damage Checkpoint Fulltext Access 11 Pages 2006
Crystal Structure of the Mitochondrial Chaperone TIM9•10 Reveals a Six-Bladed α-Propeller Fulltext Access 11 Pages 2006
Sequential ATP Hydrolysis by Cdc6 and ORC Directs Loading of the Mcm2-7 Helicase Fulltext Access 11 Pages 2006
DNA Degradation at Unprotected Telomeres in Yeast Is Regulated by the CDK1 (Cdc28/Clb) Cell-Cycle Kinase Fulltext Access 11 Pages 2006
Vertebrate POLQ and POLβ Cooperate in Base Excision Repair of Oxidative DNA Damage Fulltext Access 11 Pages 2006
Repositioning of the Reaction Intermediate within the Catalytic Center of the Spliceosome Fulltext Access 11 Pages 2006
c-Myc Phosphorylation Is Required for Cellular Response to Oxidative Stress Fulltext Access 11 Pages 2006
Regulation of Yeast NRD1 Expression by Premature Transcription Termination Fulltext Access 11 Pages 2006