Minimization of aggregation of secreted bivalent anti-human T cell immunotoxin in Pichia pastoris bioreactor culture by optimizing culture conditions for protein secretion Fulltext Access 11 Pages 2006
Development and comparison of nonradioactive in vitro kinase assays for NIMA-related kinase 2 Fulltext Access 11 Pages 2006
Calculating molar absorptivities for quinones: Application to the measurement of tyrosinase activity Fulltext Access 11 Pages 2006
Ligand binding to one-dimensional lattice-like macromolecules: Analysis of the McGhee–von Hippel theory implemented in isothermal titration calorimetry Fulltext Access 11 Pages 2006
Benefits of prolonged gradient separation for high-performance liquid chromatography–tandem mass spectrometry quantitation of plasma total 15-series F2-isoprostanes Fulltext Access 11 Pages 2006
A multiplex branched DNA assay for parallel quantitative gene expression profiling Fulltext Access 11 Pages 2006
Application of phosphoinositide-binding domains for the detection and quantification of specific phosphoinositides Fulltext Access 11 Pages 2006
Determination of cell mass and polymyxin using multi-wavelength fluorescence Fulltext Access 11 Pages 2006
The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen Fulltext Access 11 Pages 2006
Hydrolyses and transglycosylations performed by purified α-d-glucosidase of the marine mollusc Aplysia fasciata Fulltext Access 11 Pages 2006
Granule development and performance in sucrose fed anaerobic baffled reactors Fulltext Access 11 Pages 2006
Peptide, domain, and DNA affinity selection in the identification and quantitation of proteins from complex biological samples Fulltext Access 11 Pages 2006
Sol–gel encapsulation: An efficient and versatile immobilization technique for cutinase in non-aqueous media Fulltext Access 11 Pages 2006
Conversion of a CHO cell culture process from perfusion to fed-batch technology without altering product quality Fulltext Access 11 Pages 2006
Nitrification performance and microbial community dynamics in a submerged membrane bioreactor with complete sludge retention Fulltext Access 11 Pages 2006
A novel control scheme for inducing angiostatin-human IgG fusion protein production using recombinant CHO cells in a oscillating bioreactor Fulltext Access 11 Pages 2006
Molecular cloning, sequencing, expression of Chinese sturgeon cystatin in yeast Pichia pastoris and its proteinase inhibitory activity Fulltext Access 11 Pages 2006
Optimization of virus yield as a strategy to improve rabies vaccine production by Vero cells in a bioreactor Fulltext Access 11 Pages 2006
Purification and characterization of a furfural reductase (FFR) from Escherichia coli strain LYO1—An enzyme important in the detoxification of furfural during ethanol production Fulltext Access 11 Pages 2006
Retroviral vectors for vaccine development: induction of HIV-1-specific humoral and cellular immune responses in rhesus macaques using a novel MLV(HIV-1) pseudotype vector Fulltext Access 11 Pages 2006
Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: An AFM and DLS comparative study Fulltext Access 11 Pages 2006
Combining gene expression profiles and protein–protein interaction data to infer gene functions Fulltext Access 11 Pages 2006
Generic model control of the specific growth rate in recombinant Escherichia coli cultivations Fulltext Access 11 Pages 2006
Synthesis of docosahexaenoic acid derivatives designed as novel PPARγ agonists and antidiabetic agents Fulltext Access 11 Pages 2006
2D Autocorrelation modeling of the negative inotropic activity of calcium entry blockers using Bayesian-regularized genetic neural networks Fulltext Access 11 Pages 2006
Synthesis and biological evaluation of acyclic triaryl (Z)-olefins possessing a 3,5-di-tert-butyl-4-hydroxyphenyl pharmacophore: Dual inhibitors of cyclooxygenases and lipoxygenases Fulltext Access 11 Pages 2006
Immobilized methyltrioxo rhenium (MTO)/H2O2 systems for the oxidation of lignin and lignin model compounds Fulltext Access 11 Pages 2006
Synthesis and structure–activity relationship studies of 4,11-diaminonaphtho[2,3-f]indole-5,10-diones Fulltext Access 11 Pages 2006
Anticancer thiopyrano[2,3-d][1,3]thiazol-2-ones with norbornane moiety. Synthesis, cytotoxicity, physico-chemical properties, and computational studies Fulltext Access 11 Pages 2006
3-Aza-6,8-dioxabicyclo[3.2.1]octanes as new enantiopure heteroatom-rich tropane-like ligands of human dopamine transporter Fulltext Access 11 Pages 2006
Thrombomodulin induction in cultured human endothelial cells by 9-cis-locked retinoic acid analogues Fulltext Access 11 Pages 2006
Synthesis and hydrolytic behavior of two novel tripartate codrugs of naltrexone and 6β-naltrexol with hydroxybupropion as potential alcohol abuse and smoking cessation agents Fulltext Access 11 Pages 2006
Stabilization of E. coli Ribonuclease HI by the ‘stability profile of mutant protein’ (SPMP)-inspired random and non-random mutagenesis Fulltext Access 11 Pages 2006
Substituent activity relationship studies on new azolo benzoxazepinyl oxazolidinones Fulltext Access 11 Pages 2006
Novel chemo-enzymatic oligomers of cinnamic acids as direct and indirect inhibitors of coagulation proteinases Fulltext Access 11 Pages 2006
Bioactivity of glycogen phosphorylase inhibitors that bind to the purine nucleoside site Fulltext Access 11 Pages 2006
A complete set of hyaluronan fragments obtained from hydrolysis catalyzed by hyaluronidase: Application to studies of hyaluronan mass distribution by simple HPLC devices Fulltext Access 11 Pages 2006
Statistical algorithm for assuring similar efficiency in standards and samples for absolute quantification by real-time reverse transcription polymerase chain reaction Fulltext Access 11 Pages 2006
A solid-phase immunoassay of protease-resistant prion protein with filtration blotting involving sodium dodecyl sulfate Fulltext Access 11 Pages 2006
Impact of variable domain glycosylation on antibody clearance: An LC/MS characterization Fulltext Access 11 Pages 2006
SWTY—A general peptide probe for homogeneous solution binding assay of 14-3-3 proteins Fulltext Access 11 Pages 2006
Comprehensive glycan analysis of recombinant Aspergillus niger endo-polygalacturonase C Fulltext Access 11 Pages 2006
High expression level of recombinant human erythropoietin in the milk of non-transgenic goats Fulltext Access 11 Pages 2006
Coupled cytoplasmic transcription-and-translation—a method of choice for heterologous gene expression in Xenopus oocytes Fulltext Access 11 Pages 2006
Expression of the Arabidopsis feedback-insensitive anthranilate synthase holoenzyme and tryptophan decarboxylase genes in Catharanthus roseus hairy roots Fulltext Access 11 Pages 2006
Identifying causes for N2O accumulation in a lab-scale sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal Fulltext Access 11 Pages 2006
Open-loop control of the biomass concentration within the growth phase of recombinant protein production processes Fulltext Access 11 Pages 2006
The expression of recombinant human LOX-1 and identifying its mimic ligands by fluorescence polarization-based high throughput screening Fulltext Access 11 Pages 2006
Rapid concentration and purification of retrovirus by flocculation with Polybrene Fulltext Access 11 Pages 2006
Amperometric immunosensor based on toluidine blue/nano-Au through electrostatic interaction for determination of carcinoembryonic antigen Fulltext Access 11 Pages 2006
Biochemical characterization of an extracellular polygalacturonase from Trichoderma harzianum Fulltext Access 11 Pages 2006
Discrimination of Portuguese grapevines based on microsatellite markers Fulltext Access 11 Pages 2006
Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400 Fulltext Access 11 Pages 2006
On-line low-volume transesterification-based assay for immobilized lipases Fulltext Access 11 Pages 2006
Effects of mechanical stress on Anammox granules in a sequencing batch reactor (SBR) Fulltext Access 11 Pages 2006
Lipase-catalyzed synthesis of sorbitol–fatty acid esters at extremely high substrate concentrations Fulltext Access 11 Pages 2006
Total amino acid stabilization during cell-free protein synthesis reactions Fulltext Access 11 Pages 2006
Current challenges and developments in GC–MS based metabolite profiling technology Fulltext Access 11 Pages 2006
Advances in the production of sponge biomass Aplysina aerophoba—A model sponge for ex situ sponge biomass production Fulltext Access 11 Pages 2006
Bioconversion of deoxypodophyllotoxin into epipodophyllotoxin in E. coli using human cytochrome P450 3A4 Fulltext Access 11 Pages 2006
Efficient enantioselective hydrolysis of d,l-phenylglycine methyl ester catalyzed by immobilized Candida antarctica lipase B in ionic liquid containing systems Fulltext Access 11 Pages 2006
Bivalent monoclonal IgY antibody formats by conversion of recombinant antibody fragments Fulltext Access 11 Pages 2006
Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction Fulltext Access 11 Pages 2006
Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: Growth and lysine production Fulltext Access 11 Pages 2006
Encapsulation of adenoviral vectors into chitosan–bile salt microparticles for mucosal vaccination Fulltext Access 11 Pages 2006
l-Lysine biosynthetic pathway of Methylophilus methylotrophus and construction of an l-lysine producer Fulltext Access 11 Pages 2006
Mechanism of action, development and clinical experience of recombinant FVIIa Fulltext Access 11 Pages 2006
Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis Fulltext Access 12 Pages 2006
The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange Fulltext Access 12 Pages 2006
Purification, characterization, and synergistic action of phytate-resistant α-amylase and α-glucosidase from Geobacillus thermodenitrificans HRO10 Fulltext Access 12 Pages 2006
The feruloyl esterase system of Talaromyces stipitatus: Determining the hydrolytic and synthetic specificity of TsFaeC Fulltext Access 12 Pages 2006
Cloning, purification and biochemical characterization of a thrombus-ditargeting thrombolytic agent, comprised of annexin B1, ScuPA-32K and fibrin-adherent peptide Fulltext Access 12 Pages 2006
Proteomic characterization of transient expression and secretion of a stress-related metalloprotease in high cell density culture of Bacillus megaterium Fulltext Access 12 Pages 2006
Discovery, cloning and heterologous expression of secreted potato proteins reveal erroneous pre-mRNA splicing in Aspergillus oryzae Fulltext Access 12 Pages 2006
Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's “phosphate crisis” Fulltext Access 12 Pages 2006
Establishment of a real-time PCR protocol for expression studies of secondary metabolite biosynthetic gene clusters in the G/C-rich myxobacterium Sorangium cellulosum So ce56 Fulltext Access 12 Pages 2006
17β-Hydroxysteroid dehydrogenase type 7 (Hsd17b7) reverts cholesterol auxotrophy in NS0 cells Fulltext Access 12 Pages 2006
Mucosal immunization with a ricin toxin B subunit–rotavirus NSP4 fusion protein stimulates a Th1 lymphocyte response Fulltext Access 12 Pages 2006
Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis Fulltext Access 12 Pages 2006
Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations Fulltext Access 12 Pages 2006
Effect of mixing on reaction–diffusion kinetics for protein hydrogel-based microchips Fulltext Access 12 Pages 2006
Hydrolase-catalysed synthesis of peroxycarboxylic acids: Biocatalytic promiscuity for practical applications Fulltext Access 12 Pages 2006
Molecular identification of the edible ectomycorrhizal fungus Lactarius deliciosus in the symbiotic and extraradical mycelium stages Fulltext Access 12 Pages 2006
Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor Fulltext Access 12 Pages 2006
Targeted gene suppression by RNA interference: An efficient method for production of high-amylose potato lines Fulltext Access 12 Pages 2006
Diversity of plant cell wall esterases in thermophilic and thermotolerant fungi Fulltext Access 12 Pages 2006
A rapid protein expression and purification system using Chinese hamster ovary cells expressing retrovirus receptor Fulltext Access 12 Pages 2006
High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro Fulltext Access 12 Pages 2006
Discovery and investigation of a new, second triose phosphate isomerase in Klebsiella pneumoniae Fulltext Access 12 Pages 2006
Immortalized feeders for the scale-up of human embryonic stem cells in feeder and feeder-free conditions Fulltext Access 12 Pages 2006