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Molecular Biology Research Articles

FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein
Fulltext Access 9 Pages 2005
Imaging and spatial distribution of β-amyloid peptide and metal ions in Alzheimer's plaques by laser ablation-inductively coupled plasma-mass spectrometry
Fulltext Access 9 Pages 2005
Determination of methotrexate, several pteridines, and creatinine in human urine, previous oxidation with potassium permanganate, using HPLC with photometric and fluorimetric serial detection
Fulltext Access 9 Pages 2005
Isolation and mass spectrometry characterization of molecular species of lactosylceramides using liquid chromatography-electrospray ion trap mass spectrometry
Fulltext Access 9 Pages 2005
Molecular modeling and chemical modification for finding peptide inhibitor against severe acute respiratory syndrome coronavirus main proteinase
Fulltext Access 9 Pages 2005
Determination of plasma, urine, and bovine serum albumin low-molecular-weight carbonyl levels by capillary gas chromatography with electron-capture and mass-selective detection
Fulltext Access 9 Pages 2005
Control of oligonucleotide retention on a pH-stabilized strong anion exchange column
Fulltext Access 9 Pages 2005
Antibody microarrays for native toxin detection
Fulltext Access 9 Pages 2005
Rate-of-dialysis technique: Theoretical and practical aspects
Fulltext Access 9 Pages 2005
Development of an oral biosensor for salivary amylase using a monodispersed silver for signal amplification
Fulltext Access 9 Pages 2005
A fluorogenic substrate for the continuous assaying of aryl sulfatases
Fulltext Access 9 Pages 2005
Equilibrium studies of a fluorescent tacrolimus binding to surfactant protein A
Fulltext Access 9 Pages 2005
A fluorescence-based assay for fatty acid amide hydrolase compatible with high-throughput screening
Fulltext Access 9 Pages 2005
Study of primary amines for nucleophilic cleavage of cyanylated cystinyl proteins in disulfide mass mapping methodology
Fulltext Access 9 Pages 2005
Quantification of DNA strand breaks and abasic sites by oxime derivatization and accelerator mass spectrometry: Application to γ-radiation and peroxynitrite
Fulltext Access 9 Pages 2005
Quantification of yessotoxin using the fluorescence polarization technique and study of the adequate extraction procedure
Fulltext Access 9 Pages 2005
Role of pyruvate in enhancing pyruvate decarboxylase stability towards benzaldehyde
Fulltext Access 9 Pages 2005
Oligonucleotide array for detection of common severe determinants of alpha thalassemia
Fulltext Access 9 Pages 2005
Enhanced removal of organic matter and ammoniacal-nitrogen in a column experiment of tidal flow constructed wetland system
Fulltext Access 9 Pages 2005
Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101
Fulltext Access 9 Pages 2005
Efficient production of a soluble fusion protein containing human beta-defensin-2 in E. coli cell-free system
Fulltext Access 9 Pages 2005
Enhanced biosynthesis of dihydrodaidzein and dihydrogenistein by a newly isolated bovine rumen anaerobic bacterium
Fulltext Access 9 Pages 2005
Rapid cultivation of stable aerobic phenol-degrading granules using acetate-fed granules as microbial seed
Fulltext Access 9 Pages 2005
The transformation and toxicity of anthraquinone dyes during thermophilic (55 °C) and mesophilic (30 °C) anaerobic treatments
Fulltext Access 9 Pages 2005
Chemo-enzymatic synthesis of tripeptide RGD diamide in organic solvents
Fulltext Access 9 Pages 2005
Stereoselective histidine sensor based on molecularly imprinted sol-gel films
Fulltext Access 9 Pages 2005
The identification of 5′-fluoro-5-deoxyinosine as a shunt product in cell free extracts of Streptomyces cattleya
Fulltext Access 9 Pages 2005
DFT-GIAO study of aryltetralin lignan lactones: Conformational analyses and chemical shifts calculations
Fulltext Access 9 Pages 2005
Synthesis and in vitro antileishmanial activity of 5-substituted-2′-deoxyuridine derivatives
Fulltext Access 9 Pages 2005
Design and engineering of metalloproteins containing unnatural amino acids or non-native metal-containing cofactors
Fulltext Access 9 Pages 2005
Fluorophore-tagged GPCR ligands
Fulltext Access 9 Pages 2005
Chemical models for ribozyme action
Fulltext Access 9 Pages 2005
Dendrimers as artificial enzymes
Fulltext Access 9 Pages 2005
Recent advances in lipid molecular design
Fulltext Access 9 Pages 2005
Riboswitches and the role of noncoding RNAs in bacterial metabolic control
Fulltext Access 9 Pages 2005
Nucleotide analogues to investigate RNA structure and function
Fulltext Access 9 Pages 2005
Protein components for nanodevices
Fulltext Access 9 Pages 2005
Protein semisynthesis and expressed protein ligation: chasing a protein's tail
Fulltext Access 9 Pages 2005
The deletion of the pif gene improves the biosafety of the baculovirus-based technologies
Fulltext Access 9 Pages 2005
Simultaneous determination of farnesyl and geranylgeranyl pyrophosphate levels in cultured cells
Fulltext Access 9 Pages 2005
A live-cell high-throughput screening assay for identification of fatty acid uptake inhibitors
Fulltext Access 9 Pages 2005
Semiautomated cell-free conversion of prion protein: Applications for high-throughput screening of potential antiprion drugs
Fulltext Access 9 Pages 2005
A cell-based cGMP assay useful for ultra-high-throughput screening and identification of modulators of the nitric oxide/cGMP pathway
Fulltext Access 9 Pages 2005
A modified cDNA subtraction to identify differentially expressed genes from plants with universal application to other eukaryotes
Fulltext Access 9 Pages 2005
Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications
Fulltext Access 9 Pages 2005
Sequence confirmation of synthetic phosphorothioate oligodeoxynucleotides using Sanger sequencing reactions in combination with mass spectrometry
Fulltext Access 9 Pages 2005
Gravimetric antigen detection utilizing antibody-modified lipid bilayers
Fulltext Access 9 Pages 2005
Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping
Fulltext Access 9 Pages 2005
Ferrocene-based Diels-Alder derivatization for the determination of 1α-hydroxyvitamin D3 in rat plasma by high-performance liquid chromatography-electrospray tandem mass spectrometry
Fulltext Access 9 Pages 2005
Capillary electrophoresis method for the enzymatic assay of galactosyltransferases with postreaction derivatization
Fulltext Access 9 Pages 2005
Biodiesel production from triolein and short chain alcohols through biocatalysis
Fulltext Access 9 Pages 2005
Effect of calcium, phosphate and nitrogen on cell growth and biosynthesis of cell wall polysaccharides by Silene vulgaris cell culture
Fulltext Access 9 Pages 2005
Volatile and polar compounds in Rosadamascena Mill 1803 cell suspension
Fulltext Access 9 Pages 2005
Microbial adaptation to biodegradation of tert-butyl alcohol in a sequencing batch reactor
Fulltext Access 9 Pages 2005
Ethanol production from cellobiose by Zymobacter palmae carrying the Ruminocuccus albus β-glucosidase gene
Fulltext Access 9 Pages 2005
Application of response surface methodology to the modeling of α-amylase purification by aqueous two-phase systems
Fulltext Access 9 Pages 2005
Development and characterization of a monoclonal antibody directed against human telomerase reverse transcriptase (hTERT)
Fulltext Access 9 Pages 2005
Amperometric biosensor for the detection of hydrogen peroxide using catalase modified electrodes in polyacrylamide
Fulltext Access 9 Pages 2005
Lon and ClpP proteases participate in the physiological disintegration of bacterial inclusion bodies
Fulltext Access 9 Pages 2005
Differentiation of four Aspergillus species and one Zygosaccharomyces with two electronic tongues based on different measurement techniques
Fulltext Access 9 Pages 2005
Peptide mimics of epidermal growth factor (EGF) with antagonistic activity
Fulltext Access 9 Pages 2005
Effect of refeed strategies and non-ammoniagenic medium on adenovirus production at high cell densities
Fulltext Access 9 Pages 2005
Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation
Fulltext Access 9 Pages 2005
Purification, kinetic and thermodynamic studies of a new ribonuclease from a mutant of Aspergillus niger
Fulltext Access 9 Pages 2005
Fusion tags and chaperone co-expression modulate both the solubility and the inclusion body features of the recombinant CLIPB14 serine protease
Fulltext Access 9 Pages 2005
Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides
Fulltext Access 9 Pages 2005
Stereoselective lipases from Burkholderia sp., cloning and their application to preparation of methyl (R)-N-(2,6-dimethylphenyl)alaninate, a key intermediate for (R)-Metalaxyl
Fulltext Access 9 Pages 2005
Combined sulfite method for the measurement of the oxygen transfer coefficient kLa in bioreactors
Fulltext Access 9 Pages 2005
A new type of O2−-generating tool for oxidative stress studies by remodeling neutrophil NADPH oxidase
Fulltext Access 9 Pages 2005
Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterisation
Fulltext Access 9 Pages 2005
Expression of the pyranose 2-oxidase from Trametes pubescens in Escherichia coli and characterization of the recombinant enzyme
Fulltext Access 9 Pages 2005
Enhanced activity of recombinant β-secretase from Drosophila melanogaster S2 cells transformed with cDNAs encoding human β1,4-galactosyltransferase and Galβ1,4-GlcNAc α2,6-sialyltransferase
Fulltext Access 9 Pages 2005
The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells
Fulltext Access 9 Pages 2005
Heterologous expression of piglet odorant-binding protein in Pichia pastoris: a comparative structural and functional characterization with native forms
Fulltext Access 9 Pages 2005
Influence of DNA stiffness in protein-DNA recognition
Fulltext Access 9 Pages 2005
Chitin-binding domain based immobilization of d-hydantoinase
Fulltext Access 9 Pages 2005
Expression and purification of a human, soluble Arylsulfatase A for Metachromatic Leukodystrophy enzyme replacement therapy
Fulltext Access 9 Pages 2005
Lipase-catalyzed biosynthesis of cinnamoylated lipids in a selected organic solvent medium
Fulltext Access 10 Pages 2005
High-level expression and immunogenic properties of the recombinant rabbit hemorrhagic disease virus VP60 capsid protein obtained in Pichia pastoris
Fulltext Access 10 Pages 2005
Gas holdup, foaming and oxygen transfer in a jet loop bioreactor with artificial foaming media and yeast culture
Fulltext Access 10 Pages 2005
Application of Aspergillus niger-treated agrowaste residue and Glomus mosseae for improving growth and nutrition of Trifolium repens in a Cd-contaminated soil
Fulltext Access 10 Pages 2005
High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding
Fulltext Access 10 Pages 2005
Cultivation of high-rate sulfate reducing sludge by pH-based electron donor dosage
Fulltext Access 10 Pages 2005
Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization
Fulltext Access 10 Pages 2005
Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells
Fulltext Access 10 Pages 2005
Alkali and halide-resistant catalysis by the multipotent oxidase from Marinomonas mediterranea
Fulltext Access 10 Pages 2005
Development of a S. cerevisiae whole cell biocatalyst for in vitro sialylation of oligosaccharides
Fulltext Access 10 Pages 2005
Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction
Fulltext Access 10 Pages 2005
Strain development in Bacillus licheniformis: Construction of biologically contained mutants deficient in sporulation and DNA repair
Fulltext Access 10 Pages 2005
Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17β-estradiol in water samples
Fulltext Access 10 Pages 2005
Substrate specificity for the 12β-hydroxylation of bufadienolides by Alternaria alternata
Fulltext Access 10 Pages 2005
Degradation and synthesis kinetics of quorum-sensing autoinducer in Pseudomonas aeruginosa cultivation
Fulltext Access 10 Pages 2005
A rapid real-time qRT-PCR assay for ovine β-actin mRNA
Fulltext Access 10 Pages 2005
Purification and characterization of a β-d-mannosidase from the marine anaspidean Aplysia fasciata
Fulltext Access 10 Pages 2005
Yeast extract and methyl jasmonate-induced silymarin production in cell cultures of Silybum marianum (L.) Gaertn
Fulltext Access 10 Pages 2005
Cloning and expression of the malolactic gene of Pediococcus damnosus NCFB1832 in Saccharomyces cerevisiae
Fulltext Access 10 Pages 2005
Optimization of a Bifidobacterium longum production process
Fulltext Access 10 Pages 2005
Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain
Fulltext Access 10 Pages 2005
Effects of amino acid additions on ammonium stressed CHO cells
Fulltext Access 10 Pages 2005
Improving the amylolytic activity of Saccharomyces cerevisiae glucoamylase by the addition of a starch binding domain
Fulltext Access 10 Pages 2005
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