کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10148189 | 1646540 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of glutathione on sperm quality after short-term cryopreservation in seurukan fish Osteochilus vittatus (Cyprinidae)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50â¯â¯mgâ¯Lâ1. Next, the sperm thawed at 39-40â¯Â°C for 3â¯min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6â¯h after fertilization, while the hatching rate was examined 60â¯h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteochilus vittatus eggs (Pâ¯<â¯0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30â¯mgLâ1 of glutathione (63.00â¯Â±â¯5.89), however, this value was not significantly different from using concentration of 10 and 20â¯â¯mgâ¯Lâ1. A higher fertilization rate was produced using glutathione concentration of 50â¯â¯mgâ¯Lâ1 (51.33â¯Â±â¯17.01); however, it was also not significantly different from the concentration of 20, 30 and 40â¯mgLâ1. In addition, a higher hatching rate was also recorded using glutathione concentration of 50â¯mgâ¯Lâ1 (40.33â¯Â±â¯12.89), this value was not significantly different from using 40â¯mgLâ1 glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40â¯mgâ¯Lâ1 of diluent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 122, December 2018, Pages 30-34
Journal: Theriogenology - Volume 122, December 2018, Pages 30-34
نویسندگان
C.R. Muthmainnah, K. Eriani, I. Hasri, M. Irham, A.S. Batubara, Z.A. Muchlisin,