کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10153049 1666193 2018 29 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A photoelectrochemical sensor for highly sensitive detection of amyloid beta based on sensitization of Mn:CdSe to Bi2WO6/CdS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A photoelectrochemical sensor for highly sensitive detection of amyloid beta based on sensitization of Mn:CdSe to Bi2WO6/CdS
چکیده انگلیسی
A high sensitivity photoelectrochemical (PEC) immunosensor for amyloid beta (Aβ) detection, which has great neurotoxic effect on the progression of Alzheimer's disease (AD), was presented based on the sensitization of Mn2+ doped CdSe (Mn:CdSe) to Bi2WO6/CdS electrode. Bi2WO6 was synthesized successfully with a unique flower-like amorphous structure, providing a merit to load functional CdS for obtaining an expected PEC response. Mn:CdSe was used to label Aβ for acquiring Mn:CdSe-Aβ bioconjugate and enhancing the detection sensitivity via the competitive immunoreaction of Mn:CdSe-Aβ and Aβ with antibody immobilized on Bi2WO6/CdS electrode. The doped of Mn2+ in CdSe nanoparticles could induce energy defect that impeded the recombination of photo-generated charges, and greatly enhanced PEC response for ultrasensitive detection of Aβ. The proposed immunosensor for Aβ showed a linear range of 0.2 pg mL−1-50 ng mL−1 with a detection limit of 0.068 pg mL−1, also with good stability, high selectivity, and acceptable reproducibility. The sensitization of Mn:CdSe provided a new way for preparation of highly sensitive protein biosensors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 122, 30 December 2018, Pages 37-42
نویسندگان
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