کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10154487 | 1666299 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comprehensive, robust, and sensitive UPLC-MS/MS analysis of free biogenic monoamines and their metabolites in urine
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کلمات کلیدی
5-HT3-MTMAOHMAVMA5-HIAACOMTCatechol-O-methyltransferaseEPINMN3-methoxytyramine - 3-methoxythyramine5-hydroxyindole-3-acetic acid - 5-hydroxyindole-3-acetate اسیدUPLC-MS/MS - UPLC-MS / MSVanillylmandelic acid - اسید وانیلیلیماندلیکEpinephrine - اپی نفرینTobacco - توتون و تنباکوDopamine - دوپامینSerotonin - سروتونینCigarettes - سیگارMetanephrines - متانفریینmetanephrine - متانفریینnorepinephrine - نوراپی نفرینnormetanephrine - نورمنتانرینhomovanillic acid - هومووانیلیک اسیدHVA - چهcatecholamines - کاتکول امین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Biogenic monoamines, including catecholamines and serotonin are important hormones and neurotransmitters. Abnormal urinary levels of biogenic monoamines and their metabolites are associated with smoking, neuroendocrine tumors, as well as neurological and cardiovascular diseases. Measurements of free biogenic monoamines and their metabolites have been challenging because of low concentrations in complex biological matrices. Current methods require extensive enrichment and removal of interfering substances and can analyze only basic or acidic compounds in a single run. We developed a simple and robust dilute-and-shoot method capable of measuring 10 analytes, including free biogenic monoamines and their metabolites in human urine. The assay enables sensitive measurements of analytes within expected sample concentration ranges. To assess the assay's efficacy, we measured urinary levels of free biogenic monoamines and their metabolites in 255 non-smokers and 191 smokers. Our data show that while smokers had significantly higher urinary levels of free catecholamines and metanephrines, there was a decrease in levels of biogenic amine metabolites synthesized through the monoamine oxidase pathway - homovanillic acid and vanillylmandelic acid. The method could be used for high throughput measurement of the range of free biogenic amines and their metabolites in urine under a variety of different conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1099, 1 November 2018, Pages 83-91
Journal: Journal of Chromatography B - Volume 1099, 1 November 2018, Pages 83-91
نویسندگان
Zhengzhi Xie, Pawel Lorkiewicz, Daniel W. Riggs, Aruni Bhatnagar, Sanjay Srivastava,