کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10156147 | 1666375 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and characterization of bentonite/wGLP systems
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موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
ژئوشیمی و پترولوژی
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چکیده انگلیسی
Bentonite (Bent) clay is a component of soil with useful properties for enzyme adsorption. In this work, the previously characterized antioxidant superoxide dismutase (SOD) enzyme named wGLP by wheat germin-like protein was immobilized in raw (Bent) and modified bentonites (Bent-DDA). The physicochemical characterizations of both supports were carried out. Successful adsorption of wGLP onto Bent and Bent-DDA occurred on the surface and within the interlayer spacing as was revealed by X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). The release of wGLP at different times and pHs was tested. While wGLP remained almost totally immobilized into Bent-DDA up to 96â¯h, it was released from Bent to reach nearly 60% after 72â¯h at pHâ¯7.5 and preserving its SOD activity. Since tomato cell viability under the presence of Bent-wGLP was maintained, Bent-wGLP complexes are potential carriers of antioxidants in tomato cell suspension cultures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Clay Science - Volume 166, 15 December 2018, Pages 159-165
Journal: Applied Clay Science - Volume 166, 15 December 2018, Pages 159-165
نویسندگان
Andrea Y. Mansilla, Matias Lanfranconi, Vera A. Alvarez, Claudia A. Casalongué,