Production and characterization of biosurfactant from marine Streptomyces species B3

► The isolated marine Streptomyces species B3 was potential source for biosurfactant production. ► The maximum biosurfactant production was observed with sucrose and yeast extract. ► Biosurfactant reduced surface tension up to 29 mN/m, and the critical micelle concentration was 110 mg/l. ► The biosurfactant consist of 58, 33, and 8% w/w lipid, carbohydrate, and protein respectively. ► Biosurfactant was highly effective over wide range of temperature, pH, and salt concentration.