کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10377595 | 880739 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Silica nanotubes for lysozyme immobilization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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چکیده انگلیسی
Silica nanotubes were synthesized and used as enzyme immobilization carriers. The immobilization profiles were described by the adsorption of lysozyme molecules from aqueous solution onto the hydrophilic silica surface. The driving force of the adsorption, structure changes in the immobilized lysozyme molecules, and enzymatic activities were investigated. A study of the zeta potentials of silica with and without the immobilized lysozyme showed that there was an increase in the isoelectric point with the increase in the loading amount of lysozyme. FTIR spectra indicated that protein secondary structure was maintained well in the immobilized molecules. It was observed that enzymatic activities first increased and then decreased with increasing surface coverage of silica nanotubes by lysozyme, which suggested that the overlap and aggregation of lysozyme molecules reduced enzymatic activities of the adsorbed lysozyme molecules at high surface coverage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 290, Issue 1, 1 October 2005, Pages 102-106
Journal: Journal of Colloid and Interface Science - Volume 290, Issue 1, 1 October 2005, Pages 102-106
نویسندگان
Hao-Min Ding, Lei Shao, Run-Jing Liu, Qing-Gui Xiao, Jian-Feng Chen,