Fluorimetric determination of d-lactate in urine of normal and diabetic rats by column-switching high-performance liquid chromatography

The proposed HPLC method was applied to the urine of normal and diabetic rats induced by intraperitoneal administration of streptozotocin, and significant increases in d-lactate excreted into the urine were observed in diabetic rats compared to normal rats. In diabetic rats, d-lactate concentrations showed a rising tendency from the seventh day and then remained stable from the 28th day after induction, suggesting that urinary d-lactate may be used as an indicator to determine the diabetic stage and the level of kidney damage.