کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10529272 | 960792 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A method for the simultaneous determination of β-ODAP, α-ODAP, homoarginine and polyamines in Lathyrus sativus by liquid chromatography using a new extraction procedure
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The paper proposes a new method to extract β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP), α-N-oxalyl-l-α,β-diaminopropionic acid (α-ODAP), homoarginine and polyamines using 0.2 M HClO4 for only 1 h at 0-4 °C. Based on the proposed extraction, an assay is presented for simultaneously determining polyamines, the neurotoxin β-ODAP, its non-neurotoxic isomer α-ODAP and homoarginine in Lathyrus sativus extracts using a liquid chromatography (LC) system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl). The method exhibits some prominent advantages, which include: shorter extraction-time, simultaneous extraction of non-protein amino acids and polyamines and inhibition of the isomerization of β-ODAP to α-ODAP. The assay proved satisfactory for the quantitative determination of L. sativus extracts of seeds and seedlings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 534, Issue 2, 8 April 2005, Pages 199-205
Journal: Analytica Chimica Acta - Volume 534, Issue 2, 8 April 2005, Pages 199-205
نویسندگان
Ze yi Yan, Cheng jin Jiao, Yang peng Wang, Feng min Li, Yong min Liang, Zhi xiao Li,