Functional annotation and characterization of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1

► Flavoprotein hydroxylases from R. jostii RHA1 have been functionally annotated. ► Mis-annotated Q0SFK6 encodes for 3-hydroxybenzoate 6-hydroxylase (3HB6H). ► 3HB6H is a homodimer. ► 3HB6H converts ortho- and meta-substituted 3-hydroxybenzoates. ► Gentisate is a good effector of 3HB6H.