S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana

Nitric oxide is produced in vast amounts during plant-pathogen interaction, potentially targeting key regulatory factors in plants, such as AtMYB30 transcription factor. Indeed, NO modification of C49 and C53 residues promotes a subtle modification on the secondary structure of AtMYB30. Using various techniques, the direct effect of NO in AtMYB30, and thus the potential consequences of NO activity on plant metabolism influenced by this transcription factor were demonstrated in this work.145