High operational stability of invertase from Saccharomyces cerevisiae immobilized on chitosan nanoparticles

► Chitosan nanoparticles were prepared for enzyme immobilization. ► Invertase from S. cerevisiae was covalently immobilized on chitosan nanoparticles. ► The support provided high superficial area. ► The immobilization yield was 74.3% and immobilization efficiency 61.6%. ► High operational stability was reached: 59 reuses with maximal enzyme activity.