Polyplex-microbubble hybrids for ultrasound-guided plasmid DNA delivery to solid tumors

Polyplex-microbubbles were generated by covalently attaching thiolated PEG-PEI to maleimide-microbubbles and mixing with plasmid DNA. The polyplex-microbubbles circulated systemically and, with applied ultrasound, successfully transfected tumor tissue in vivo.311