کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738083 | 1046682 | 2011 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lipid hydroperoxide-induced and hemoglobin-enhanced oxidative damage to colon cancer cells
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کلمات کلیدی
ECDFPGHNEdichlorofluoresceinDDEDNA adducts1O2MRMEndonuclease IIIDcfEndoIII4-hydroxynonenalTrans,trans-2,4-decadienalH2DCF-DAMDA8-oxodGuodGuo2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetate2′-deoxyguanosine - 2'-deoxyguanosine8-oxo-7,8-dihydro-2′-deoxyguanosine - 8-اکسو-7،8-دی هیدرو-2'-دگزسی گوانوزینDNA damage - آسیبDNASinglet molecular oxygen - اکسیژن مولکولی تک سلولیElectrochemical detection - تشخیص الکتروشیمیاییOxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادColorectal cancer - سرطان روده بزرگElectrospray ionization tandem mass spectrometry - طیف سنجی جرمی یونیزه الکترو اسپری یونیزاسیونformamidopyrimidine DNA glycosylase - فرمامیدوپیریمیدین دی ان ای گلیکوزیلازmalonaldehyde - مالونالدئیدmultiple reaction monitoring - نظارت چندگانه چندگانهHemoglobin - هموگلوبینLinoleic acid hydroperoxide - هیدروپراکسید اسید لینولئیکLipid hydroperoxide - هیدروپراکسید لیپیدCRC - کد افزونگی دورهای high-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Epidemiological studies have indicated that Western diets are related to an increase in a series of malignancies. Among the compounds that are credited for this toxic effect are heme and lipid peroxides. We evaluated the effects of hemoglobin (Hb) and linoleic acid hydroperoxides (LAOOH) on a series of toxicological endpoints, such as cytotoxicity, redox status, lipid peroxidation, and DNA damage. We demonstrated that the preincubation of SW480 cells with Hb and its subsequent exposure to LAOOH (Hb + LAOOH) led to an increase in cell death, DCFH oxidation, malonaldehyde formation, and DNA fragmentation and that these effects were related to the peroxide group and the heme present in Hb. Furthermore, Hb and LAOOH alone exerted a toxic effect on the endpoints assayed only at concentrations higher than 100 μM. We were also able to show that SW480 cells presented a higher level of the modified DNA bases 8-oxo-7,8-dihydro-2â²-deoxyguanosine and 1,N2-etheno-2â²-deoxyguanosine compared to the control. Furthermore, incubations with Hb led to an increase in intracellular iron levels, and this high level of iron correlated with DNA oxidation, as measured as EndoIII- and Fpg-sensitive sites. Thus, Hb from either red meat or bowel bleeding could act as an enhancer of fatty acid hydroperoxide genotoxicity, which contributes to the accumulation of DNA lesions in colon cancer cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 51, Issue 2, 15 July 2011, Pages 503-515
Journal: Free Radical Biology and Medicine - Volume 51, Issue 2, 15 July 2011, Pages 503-515
نویسندگان
José Pedro F. Angeli, Camila Carrião M. Garcia, Fernanda Sena, Florencio P. Freitas, Sayuri Miyamoto, Marisa Helena G. Medeiros, Paolo Di Mascio,