کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10751838 | 1050319 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular dynamics simulations of Na+ and leucine transport by LeuT
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Molecular dynamics simulations are used to gain insight into the binding of Na+ and leucine substrate to the bacterial amino acid transporter LeuT, focusing on the crystal structures of LeuT in the outward-open and inward-open states. For both conformations of LeuT, a third Na+ binding site involving Glu290 in addition to the two sites identified from the crystal structures is observed. Once the negative charge from Glu290 in the inward-open LeuT is removed, the ion bound to the third site is ejected from LeuT rapidly, suggesting that the protonation state of Glu290 regulates Na+ binding and release. In Clâ-dependent transporters where Glu290 is replaced by a neutral serine, a Clâ ion would be required to replace the role of Glu290. Thus, the simulations provide insights into understanding Na+ and substrate transport as well as Clâ-independence of LeuT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 464, Issue 1, 14 August 2015, Pages 281-285
Journal: Biochemical and Biophysical Research Communications - Volume 464, Issue 1, 14 August 2015, Pages 281-285
نویسندگان
Rong Chen, Shin-Ho Chung,