کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754758 | 1050361 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Snail interacts with hPLSCR1 promoter and down regulates its expression in IMR-32
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کلمات کلیدی
PLSTFsepithelial mesenchymal transition - انتقال مزانشیمی epithelialchromatin immunoprecipitation - ایمن سازی کروماتینTranscriptional regulation - تنظیم ترانزیتیEMT - تکنسین فوریتهای پزشکیSnail - حلزونTranscription factors - عوامل رونویسیPhosphatidylserine - فسفاتیدیلسرینPhospholipids - فسفولیپیدPromoter - پروموترCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Human phospholipid scramblase 1 (hPLSCR1) is a proapoptotic protein whose expression is deregulated in a variety of cancers cells. However till date the transcription regulation of hPLSCR1 is unknown. Transcriptional regulation of hPLSCR1 was studied by cloning the 5â²-flanking region of hPLSCR1. Luciferase assays revealed that â1525 to â1244 region of hPLSCR1 was found to regulate its promoter activity. A putative Snail transcription factor (TF) binding site was found within the regulatory region of the promoter. Snail binding was found to down regulate the expression of hPLSCR1 both at the transcriptional and translational levels. Snail knock down using Snail-shRNA confirmed that down regulation of hPLSCR1 by Snail was specific. Point mutation studies confirm that the predicted Snail TF binds to â1123 to â1117 site. ChIP assay further confirms the physical interaction of Snail with hPLSCR1 promoter. This is the first report showing the transcriptional regulation of hPLSCR1 expression by Snail TF and its possible implications in cancer progression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 1, 18 July 2014, Pages 172-177
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 1, 18 July 2014, Pages 172-177
نویسندگان
Vincent Gerard Francis, Purnima Padmanabhan, Sathyanarayana N. Gummadi,