Positive feedback control between STIM1 and NFATc3 is required for C2C12 myoblast differentiation

► Increased expression of NFATc3 and STIM1 is required for C2C12 myoblast differentiation. ► NFATc3 regulates STIM1 expression, SOCE activity, and myotube formation. ► STIM1 regulates NFATc3 expression and its activity, and myotube formation. ► Positive feedback control between STIM1 and NFATc3 is required for myoblast differentiation.