PKC and RhoA signals cross-talk in Escherichia coli endotoxin induced alterations in brain endothelial permeability

► We addressed how LPS regulates blood-brain barrier permeability by disrupting the tight junction. ► PKC (a, β, ζ) and RhoA invovled, but PI3 K and tyrosine kinase not involved in the regulatory process. ► PKC-a and PKC-ζ, but not PKC-β interacted with RhoA in Bend.3 cells stimulated by LPS. ► PKC-a and PKC-ζ separately acted as the upstream and downstream molecule for Rho. ► It may play a role in understanding the mechanism of meningeal inflammation.