Quantitative multiplex quantum dot in-situ hybridisation based gene expression profiling in tissue microarrays identifies prognostic genes in acute myeloid leukaemia

► Development of a quantitative high throughput in situ expression profiling method. ► Application to a tissue microarray of 242 AML bone marrow samples. ► Identification of HOXA4, HOXA9, Meis1 and DNMT3A as prognostic markers in AML.