Influence of divalent cations on the cytoskeletal dynamics of K562 cells determined by nano-scale bead tracking

► Nano-scale bead tracking is a robust method to measure the reorganization dynamics of cellular cytoskeletal structures. ► The directionality of the bead motion is influenced by the overall stability of the cytoskeletal network in K562 cells. ► The persistence is not affected by the binding of either Mn2+ or Mg2+/Ca2+ ions.