Housekeeping gene expression in myogenic cell cultures from neurodegeneration and denervation animal models

► We examined housekeeping gene expression stability in mouse myogenic cell cultures. ► We determined the influence of sex, muscle-type, age and pathology in the housekeeping expression. ► The most influencing factor is the age of the donor. ► Rn18s gene should be avoided as normalization factor. ► The inclusion of two of the genes is enough to create a reliable normalization factor.