Distinct functions of human MVB12A and MVB12B in the ESCRT-I dependent on their posttranslational modifications

► Human MVB12s do not function as a stalk region component of the ESCRT-I complex. ► MVB12B is a ubiquitinated protein, and MVB12A has a ubiquitin-binding site. ► The ubiquitinations sites in MVB12B are Lys264 and Lys290. ► MVB12B may be normally suppressed through the ubiquitin-proteasome pathway.