کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10769358 | 1050821 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding and regulation of hypoxia-inducible factor-1 by the inhibitory PAS proteins
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Hypoxia-inducible factor-1 (HIF-1), which consists of oxygen-sensitive HIF-1α and constitutively expressed HIF-1β subunits, activates transcription of genes encoding proteins that mediate adaptive responses to reduced oxygen availability. The mouse inhibitory PAS (Per/Arnt/Sim) domain protein (IPAS) functions as a negative regulator in HIF-mediated gene expression. In this report, we cloned the human orthologs of the mouse IPAS gene, IPASH1 and IPASH2, to further study the regulatory mechanism of HIF-1 by the IPAS proteins. The human IPAS proteins inhibited the transactivation function of HIF-1α under hypoxic conditions. In addition, human IPAS proteins blocked the hypoxia-induced VEGF expression and inhibited cell migration and tube formation of human umbilical vein endothelial cells. Interestingly, both HIF-1α and HIF-1β interacted with the IPAS proteins. Collectively, these results suggest that human IPAS proteins inhibit angiogenesis by binding to and inhibiting HIF-1α and HIF-1β.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 1, 11 November 2005, Pages 209-215
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 1, 11 November 2005, Pages 209-215
نویسندگان
Mi Sun Jang, Jae Eun Park, Jung A. Lee, Sung Goo Park, Pyung Keun Myung, Do Hee Lee, Byoung Chul Park, Sayeon Cho,