کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803203 | 1055803 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Uptake of LPS/E. coli/latex beads via distinct signalling pathways in medfly hemocytes: the role of MAP kinases activation and protein secretion
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کلمات کلیدی
E. coliLPSJnkERKGRB2c-Jun N-terminal kinase - C-Jun N-terminal kinaseMAPK - MAPKInsect immunity - ایمنی حشرهProtein secretion - ترشح پروتئینextracellular-signal regulated kinase - سیگنال تنظیم شده سیگنال خارج سلولیPhagocytosis - فاگوسیتوز یا بیگانهخواری MEK - مجاهدین خلقmitogen-activated protein kinase - پروتئین کیناز فعال با mitogengrowth factor receptor-bound protein 2 - پروتئین گیرنده پروتئینی 2 عامل رشدMAP kinase - کیناز MAP
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In response to LPS/E. coli treatment, extracellular signal-regulated kinase (ERK) is activated in medfly hemocytes. To explore the molecular mechanisms underlying LPS/E. coli/latex beads endo- and phagocytosis, we studied the signalling pathways leading to p38 and c-jun N-terminal kinase (JNK) activation. JNK and p38-like proteins were initially identified within medfly hemocytes. Flow cytometry analysis revealed that mitogen-activated protein kinases (MAPK) are required for phagocytosis. Inhibition of specific MAPK signalling pathways, with manumycin A, toxin A, cytochalasin D and latrunculin A, revealed activation of p38 via Ras/Rho/actin remodelling pathway and activation of JNK that was independent of actin cytoskeleton reorganization. ERK and p38 pathways, but not JNK, appeared to be involved in LPS-dependent hemocyte secretion, whereas all MAPK subfamilies seemed to participate in E. coli-dependent secretion. In addition, flow cytometry experiments in hemocytes showed that the LPS/E. coli-induced release was a prerequisite for LPS/E. coli uptake, whereas latex bead phagocytosis did not depend on hemocyte secretion. This is a novel aspect, as in mammalian monocytes/macrophages LPS/E. coli-triggered release has not been yet correlated with phagocytosis. It is of interest that these data suggest distinct mechanisms for the phagocytosis of E. coli and latex beads in medfly hemocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1744, Issue 1, 15 May 2005, Pages 1-10
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1744, Issue 1, 15 May 2005, Pages 1-10
نویسندگان
Irene Lamprou, Sotiris Tsakas, Georgios L. Theodorou, Marina Karakantza, Maria Lampropoulou, Vassilis J. Marmaras,