Effects of kisspeptin-10 on progesterone secretion in cultured chicken ovarian granulosa cells from preovulatory (F1-F3) follicles

► Kisspeptin immunoreactive substance is expressed in chicken ovarian granulosa cells. ► Kisspeptin-10 (Kp-10) stimulates progesterone secretion in these cells from preovulatory (F1-F3) follicles. ► Stimulated progesterone secretion parallels upregulation of StAR, P450scc and 3β-HSD mRNA expression.