HIV-1 envelope trimer has similar binding characteristics for carbohydrate-binding agents as monomeric gp120

► Similar binding kinetics of CBAs for monomeric gp120 and trimeric gp140. ► Similar binding kinetics of the Ab 2G12 and sCD4 for monomeric gp120 and trimeric gp140. ► Two- to threefold stronger affinity of Ab b12 and DC-SIGN for trimeric gp140. ► SPR-directed binding kinetics with monomeric gp120 are relevant for native gp140 trimer.