The crystal structure of galactitol-1-phosphate 5-dehydrogenase from Escherichia coli K12 provides insights into its anomalous behavior on IMAC processes

► rGPDH from E. coli spontaneously interact with NTA matrices. ► rGPDH is a homogeneous, stable dimer in solution. ► The presence of an N-terminal His6-tag in GPDH has adverse effects in its stability. ► rGPDHis an NAD(H)-dependent zinc metalloenzyme. ► The crystal structure of rGPDH has been solved up to 1.87 Å resolution.