H3K27 methylation and H3S28 phosphorylation-dependent transcriptional regulation by INHAT subunit SET/TAF-Iβ

► Binding profiles of SET/TAF-Iβ to variously modified histone peptides. ► SET/TAF-Iβ strongly recognized PRC2-mediated H3K27me1/2/3. ► SET/TAF-Iβ binding to H3K27me1/2/3 was completely disrupted by H3S28P. ► SET/TAF-Iβ sequentially recruited to ATF3 promoter after PRC2 complex.