کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10876995 | 1075898 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Micropropagation of Dendrobium nobile from shoot tip sections
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Successful shoot regeneration of Dendrobium nobile was achieved using thin shoot tip sections and triacontanol (TRIA) for the first time. Protocorm-like bodies (PLBs) or proliferating shoot buds were observed when thin shoot tip sections were cultured on the basal medium of Mitra et al. (Indian J. Exp. Biol. 14 (1976) 350) supplemented with 4.0 μg Lâ1 TRIA. The highest percentage of explants (93%) produced PLBs or proliferating shoot buds (21) at 4.0 μg Lâ1 TRIA-supplemented basal medium. All the newly formed PLBs or proliferating shoot buds survived and ultimately produced healthy shoots with 2-3 leaves. Shoots produced roots when cultured on basal medium supplemented with 2.0 μg Lâ1 TRIA. The well-rooted shoots were transferred to pots containing charcoal chips, coconut husk and broken tiles (2:2:1), and a 92% survival rate was achieved. This work reveals that TRIA can be used as an effective growth regulator in the micropropagation and conservation of D. nobile.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 162, Issue 4, 22 April 2005, Pages 473-478
Journal: Journal of Plant Physiology - Volume 162, Issue 4, 22 April 2005, Pages 473-478
نویسندگان
Ravindra B. Malabadi, Gangadhar S. Mulgund, Nataraja Kallappa,