کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899513 | 1084383 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ROBO3 promotes growth and metastasis of pancreatic carcinoma
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کلمات کلیدی
miRNAssFRPROBO3TGF-βPDACEndothelial to mesenchymal transition - Endothelial به انتقال مزانشیمیPancreatic ductal adenocarcinoma - آدنوکارسینوم پانکراس داکتالtransforming growth factor-β - تبدیل فاکتور رشد βInvasion - تهاجمEMT - تکنسین فوریتهای پزشکیmicroRNAs - ریز آرانایPancreatic cancer - سرطان پانکراسMetastasis - متاستاز Secreted frizzled related protein - پروتئین مربوط به فروتوزاسیون ترشح می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pancreatic carcinoma is a highly lethal malignancy with an extremely poor prognosis. Recent genome-wide studies have implicated axon guidance pathways, including the SLIT/ROBO pathway, in pancreatic tumor development and progression. Here we showed that ROBO3 expression is up-regulated in pancreatic cancer tissue samples and cell lines. Over-expression of ROBO3 promotes pancreatic cancer cell growth, invasion and metastasis in vitro and in mouse xenograft tumor models. We identified miR-383 as a suppressor of ROBO3, and revealed its expression to be inversely correlated with ROBO3. Over-expression of ROBO3 activates Wnt pathway components, β-catenin and GSK-3, and the expression of markers indicating an EMT. By means of immunoprecipitation, we revealed an interaction between Wnt inhibitor SFRP and ROBO3 in pancreatic cancer cell lines. Our work suggests that ROBO3 may contribute to the progression of pancreatic cancer by sequestering Wnt inhibitor SFRP, which in turn leads to increased Wnt/β-catenin pathway activity. We also confirmed that ROBO3 increases with clinical grade and miR-383 expression is inversely correlated to that of ROBO3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 366, Issue 1, 28 September 2015, Pages 61-70
Journal: Cancer Letters - Volume 366, Issue 1, 28 September 2015, Pages 61-70
نویسندگان
Shilong Han, Chuanwu Cao, Tao Tang, Chenhui Lu, Jichong Xu, Shi Wang, Lei Xue, Xiaoping Zhang, Maoquan Li,