Nkx6 genes pattern the frog neural plate and Nkx6.1 is necessary for motoneuron axon projection

► We have investigated Nkx6 function in the amphibian neural plate. ► Nkx6.1 and Nkx6.2 mRNA injections inhibit primary neurogenesis and intermediate neural plate markers. ► Nkx6.1 antisense morpholino oligonucleotide injected tadpoles are paralyzed. ► Nkx6.1 MO injected tadpoles fail to extend motor axons.