کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11000459 | 1423875 | 2019 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis and chemical composition analysis of protocatechualdehyde-based novel melanin dye by 15T FT-ICR: High dyeing performance on soft contact lens
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, novel melanin pigments were prepared through the eco-friendly microbial conversion of caffeic acid by engineering Escherichia coli BL21(DE3) cells. The cells expressed feruloyl-CoA synthetase (FCS) and enoyl-CoA hydratase/aldolase (ECH) for the conversion of caffeic acid into protocatechualdehyde. The synthesized protocatechualdehyde further underwent oxidative modification into a diquinone structure, followed by polymerization into melanin complex (F/E-Melanin). The expression of FCS and ECH in the presence of 90â¯mg/L caffeic acid (0.5â¯mM) could produce F/E-Melanin black pigments up to 170â¯mg dry wt/L within 12â¯h. Interestingly, the synthesized melanin was applied for hydrogel of soft contact lens dyeing and showed excellent dyeing performance with biological activities of antibacterial and antioxidant capacities, as well as a higher water-content rate than those of the existing synthetic melanins prepared in this study. The synthesized F/E-Melanin polymers were structurally analyzed by 1H NMR and FT-IR. Interestingly, the molecular composition such as Van Krevelen plots with H/C and O/C ratio, double bond equivalents with carbon number, and CHONS distribution of the five synthetic melanins were characterized by interpreting data obtained from 15T FT-ICR mass spectrometry analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 160, January 2019, Pages 546-554
Journal: Dyes and Pigments - Volume 160, January 2019, Pages 546-554
نویسندگان
Soo-Yeon Ahn, Mira Choi, Da-woon Jeong, SeoA. Park, HyunA. Park, Kyoung-Soon Jang, Kwon-Young Choi,