کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11020257 | 1717600 | 2019 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of total and free ceftolozane and tazobactam in human plasma and interstitial fluid by HPLC-UV
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Ceftolozane/tazobactam is a new cephalosporin/beta-lactamase inhibitor combination. An HPLC-UV method is described for the determination of total and free ceftolozane and tazobactam in human plasma and in microdialysate of subcutaneous tissue, respectively. Separation was performed using a reversed-phase column with phosphate buffer/acetonitrile as eluent and photometric detection at 260ânm (ceftolozane) or 220ânm (tazobactam). Linearity has been shown down to ceftolozane/tazobactam 0.1/0.05âmg/L in plasma and 0.03/0.015âmg/L in saline, respectively. The plasma protein binding of both drugs as determined by ultrafiltration was less than 10%. Temperature, pH or relative centrifugation force (up to 3000 x g) had no significant impact on the protein binding. The method was applied to the determination of ceftolozane and tazobactam in plasma and interstitial fluid of healthy volunteers following intravenous infusion of ceftolozane/tazobactam 1.0/0.5âg.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 163, 30 January 2019, Pages 34-38
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 163, 30 January 2019, Pages 34-38
نویسندگان
Alexander Kratzer, Selina SchieÃer, Peter Matzneller, Beatrix Wulkersdorfer, Markus Zeitlinger, Jens Schlossmann, Frieder Kees, Christoph Dorn,