کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11263085 | 1788350 | 2019 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chicken GHR antisense transcript regulates its sense transcript in hepatocytes
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کلمات کلیدی
CCK-8UTRRT-Q-PCRdouble strand RNAWRRRNaseIGFslncRNAsE16GHRDAPIRT-PCRASOgDNAcDNA - cDNADNA complementary to RNA - DNA مکمل RNAEdU - EDUNATs - NAT هاAntisense oligodeoxynucleotide - oligodeoxynucleotide antisensecircular RNAs - RNA های دایره ایlong noncoding RNAs - RNA های غیر Coding طولانیsld - SldExon - اکسونStability - ثباتBase pair(s) - جفت پایه (ها)Natural antisense transcripts - رونوشت های آنتیسنس طبیعیreverse transcription PCR - رونویسی معکوس PCRribonuclease - ریبونوکلئازReal time quantitative PCR - زمان واقعی PCR کمیhours - ساعت هاcircRNAs - سیرک هاceRNA - سینهcell counting kit-8 - شمارش سلول کیت 8insulin-like growth factors - عوامل رشد انسولین مانندnatural antisense transcript - متن انسیسیس طبیعیMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAGrowth hormone - هورمون رشدgrowth hormone receptor - گیرنده هورمون رشد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
An increasing number of evidences indicated that long noncoding RNAs (LncRNAs) regulate a variety of biological progresses via different mechanisms. Our previous study had identified a chicken growth hormone receptor (GHR) antisense transcript (GHR-AS) which regulated GHR sense transcript (GHR-S) in LMH cells. In the present study, roles of GHR-AS and its regulatory mechanism were analyzed in chicken hepatocytes. The expression patterns of liver GHR-S, GHR-AS and Let-7b ascended with the development of chicken. The hepatocytes proliferation was promoted and more cells entered into DNA synthesis (S) phase when GHR-AS was overexpressed while the cell proliferation was slowed and fewer cells were in S phase when GHR-AS was interfered. Meanwhile, the GHR-S increased when we overexpressed GHR-AS while it reduced when GHR-AS was inhibited. The S1 Nuclease protection assay indicated that GHR-S and GHR-AS formed RNA duplex via GHR-S 3â² untranslation regon (3â²UTR). In hepatocytes or LMH cells, the half-time of GHR-S showed a delayed trend when GHR-AS or GHR-AS 5â² untranslation regon (5â²UTR) was overexpressed. Furthermore, the level of GHR-S can be decreased by Let-7b mimics whereas it was partially rescued when co-transfected pGHR-AS or pGHR-AS 5â²UTR with Let-7b mimics. Based on our findings, GHR-AS affected hepatocytes proliferation and improved GHR-S stability possibly by forming RNA duplex between GHR-S and GHR-AS, competing with Let-7b.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 682, 15 January 2019, Pages 101-110
Journal: Gene - Volume 682, 15 January 2019, Pages 101-110
نویسندگان
Zhang Wang, HaiDong Xu, Ting Li, Jiang Wu, LiLong An, ZhiHui Zhao, Mei Xiao, Patricia Adu-Asiamah, XiQuan Zhang, Li Zhang,