Current trends in separation of plasmid DNA vaccines: A review

Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid.

Strategies for purifying supercoiled plasmid DNA.Figure optionsDownload as PowerPoint slideHighlights
► Current trends in the separation and purification of Plasmid DNA vaccines.
► Practical large and analytical scale productions of the plasmid DNA vaccine are discussed.
► Separation challenges.
► New developments and process solutions.
► Future prospective in the field of plasmid DNA vaccines.