A new method for determination of myricetin and quercetin using solid phase microextraction–high performance liquid chromatography–ultra violet/visible system in grapes, vegetables and red wine samples

A new approach for the extraction and determination of myricetin and quercetin by using SPME–HPLC–UV system has been developed. The method involves adsorption of flavonoids on CAR/TPR fiber followed by desorption in the desorption chamber of SPME–HPLC interface using citrate buffer (0.001 M):acetonitrile (70:30) as mobile phase and UV detection at 372 nm. The detection limits for myricetin and quercetin are 48.3 and 24.7 pg mL−1, respectively. The proposed method was validated by determining myricetin and quercetin in tomato, onion, grapes and red wine samples.