کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1172432 | 960768 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Directly light scattering imaging of the aggregations of biopolymer bound chromium(III) hydrolytic oligomers in aqueous phase and liquid/liquid interface
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Investigations of inorganic oligomers are important in both chemistry and physiology. In this contribution, we propose a laser induced light scattering imaging (LSI) and a total internal reflected light scattering imaging (TIR-LSI) technique, and apply them to characterize the interactions of inorganic oligomers with biopolymer in aqueous phase and at liquid/liquid interface, respectively. In aqueous medium, synthetic chromium(III) hydrolytic oligomers (CrHO) react with DNA, and the resultant binary could be extracted into the H2O/CCl4 interface in the presence of triocyctyl phosphine oxide (TOPO), forming a DNA-CrHO-TOPO ternary amphipathic complex at the interface with the associate constant of 1.32 Ã 103 molâ1 dm4 for a given 1.0 Ã 10â4 mol lâ1 TOPO. Under the excitation of a 441-nm He-Cd laser light beam, the resultant light scattering and total internal reflected light scattering (TIR-LS) signals of the formed binary in aqueous phase and ternary at liquid/liquid interface could be easily captured using a common microscope coupled with a CCD camera. By digitally analyzing the CCD captures, we demonstrate that aggregations of the CrHO-DNA binary in aqueous phase and DNA-CrHO-TOPO ternary at liquid/liquid interface have occurred, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 567, Issue 2, 17 May 2006, Pages 143-151
Journal: Analytica Chimica Acta - Volume 567, Issue 2, 17 May 2006, Pages 143-151
نویسندگان
Jian Ling, Cheng Zhi Huang, Yuan Fang Li,