کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1178206 962674 2016 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Physiological enzymology: The next frontier in understanding protein structure and function at the cellular level
ترجمه فارسی عنوان
آنزیم شناسی فیزیولوژیکی: مرز بعدی در درک ساختار پروتئین و عملکرد در سطح سلولی
کلمات کلیدی
آنزیم شناسی؛ سینتیک؛ طیف سنجی؛ ساختار پروتئین؛ تک مولکول
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Provides a brief history of classical techniques used in enzymology
• Discusses current techniques used in chemical and synthetic biology
• Describes contents of Special Issue related to methods in physiological enzymology

Historically, the study of proteins has relied heavily on characterizing the activity of a single purified protein isolated from other cellular components. This classic approach allowed scientists to unambiguously define the intrinsic kinetic and chemical properties of that protein. The ultimate hope was to extrapolate this information toward understanding how the enzyme or receptor behaves within its native cellular context. These types of detailed in vitro analyses were necessary to reduce the innate complexities of measuring the singular activity and biochemical properties of a specific enzyme without interference from other enzymes and potential competing substrates. However, recent developments in fields encompassing cell biology, molecular imaging, and chemical biology now provide the unique chemical tools and instrumentation to study protein structure, function, and regulation in their native cellular environment. These advancements provide the foundation for a new field, coined physiological enzymology, which quantifies the function and regulation of enzymes and proteins at the cellular level. In this Special Edition, we explore the area of Physiological Enzymology and Protein Function through a series of review articles that focus on the tools and techniques used to measure the cellular activity of proteins inside living cells. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1864, Issue 1, January 2016, Pages 65–69
نویسندگان
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