کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1178705 962713 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High throughput characterization of structural differences between closely related proteins in solution
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High throughput characterization of structural differences between closely related proteins in solution
چکیده انگلیسی

Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired.

Figure optionsDownload high-quality image (156 K)Download as PowerPoint slideHighlights
► Distinguishing protein isoforms without detailed structural analysis is often needed.
► Analytical tool exploiting isoform differences in solvent interaction is introduced.
► This technique is based on the protein partition in aqueous two-phase systems (ATPS).
► ATPS generate very crowded environment.
► Macromolecular crowding conditions of ATPS amplify structural differences of isoforms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1834, Issue 2, February 2013, Pages 583–592
نویسندگان
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