کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1196298 964555 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calibration Function for the Orbitrap FTMS Accounting for the Space Charge Effect
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Calibration Function for the Orbitrap FTMS Accounting for the Space Charge Effect
چکیده انگلیسی

Ion storage in an electrostatic trap has been implemented with the introduction of the Orbitrap Fourier transform mass spectrometer (FTMS), which demonstrates performance similar to high-field ion cyclotron resonance MS. High mass spectral characteristics resulted in rapid acceptance of the Orbitrap FTMS for Life Sciences applications. The basics of Orbitrap operation are well documented; however, like in any ion trap MS technology, its performance is limited by interactions between the ion clouds. These interactions result in ion cloud couplings, systematic errors in measured masses, interference between ion clouds of different size yet with close m/z ratios, etc. In this work, we have characterized the space-charge effect on the measured frequency for the Orbitrap FTMS, looking for the possibility to achieve sub-ppm levels of mass measurement accuracy (MMA) for peptides in a wide range of total ion population. As a result of this characterization, we proposed an m/z calibration law for the Orbitrap FTMS that accounts for the total ion population present in the trap during a data acquisition event. Using this law, we were able to achieve a zero-space charge MMA limit of 80 ppb for the commercial Orbitrap FTMS system and sub-ppm level of MMA over a wide range of total ion populations with the automatic gain control values varying from 10 to 107.

Graphical AbstractTwo-term calibration equation for the Orbitrap FTMS that takes into account space charge effect on the frequency of ions' motion.Figure optionsDownload high-quality image (111 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 21, Issue 11, November 2010, Pages 1846–1851
نویسندگان
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