کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1205265 | 1493662 | 2012 | 7 صفحه PDF | دانلود رایگان |
The retention properties of a silica gel column and a type C silica (silicon hydride) column for bases, sugars and polar acids were compared in hydrophilic interaction chromatography (HILIC) mode with formic acid or ammonium acetate as aqueous phase modifiers. The type C silica column was much more retentive for a series of model bases than the silica gel column and, surprisingly, retention of bases increased on the type C silica column when, the higher pH, ammonium acetate containing mobile phase was used. The retention of sugars was greater on the type C silica column than on the silica gel column and also increased on the type C silica column with increased pH suggesting either a silanophilic mechanism of retention or some unknown mechanism. Three type C silica based columns, type C silica, cogent diamond hydride and a β-pinene modified column, which it was hoped might exert some additional stereochemical discrimination, were tested for metabolomic profiling of urine. In general the unmodified type C silica column gave the strongest retention of the many polar metabolites in urine and could provide a useful complement to established HILIC methods for metabolomic profiling.
► Reports the first fundamental comparison of chromatography on silica gel and type C silica.
► Makes some surprising observations on the behaviour of bases and sugars on type C silica.
► Reports the synthesis of a pinene modified type C silica.
► Compares the behaviour of three type C silica columns for metabolic profiling.
Journal: Journal of Chromatography A - Volume 1263, 9 November 2012, Pages 61–67