کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1208439 | 1493730 | 2008 | 5 صفحه PDF | دانلود رایگان |
Vigabatrin is widely used as an anticonvulsant in the treatment of seizures. Vigabatrin is usually supplied as racemate in formulation, but only the (S)-(+)-enantiomer of vigabatrin is pharmacologically active. A simple and sensitive liquid chromatographic method is described for the separation and quantification of vigabatrin enantiomers. The method is based on derivatizing racemic vigabatrin with a fluorescent chiral reagent (naproxen acyl chloride). The resulting diastereomeric derivatives are highly responsive to a fluorimetric detector (λex = 230 nm, λem = 350 nm). The lower quantitation limit of the method is attainable at 25 nM for (S)-(+)-vigabatrin or (R)-(−)-vigabatrin with a detection limit of about 2.5 nM (S/N = 3 with 10 μl injected). Application of the method to the analysis of vigabatrin in serum of dosed patients proved feasible.
Journal: Journal of Chromatography A - Volume 1178, Issues 1–2, 18 January 2008, Pages 166–170