کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212333 | 1494066 | 2015 | 6 صفحه PDF | دانلود رایگان |
• Arginine ligand selectivity combined with monoliths versatility.
• Optimization of sc pDNA HPV-16 E6/E7 purification by design of experiments.
• Multiple interactions promoted by the combination of binding step, washing step and pH.
• Improvement of sc pDNA recovery from 39% to 83%, maintaining 100% of purity.
The progress of DNA vaccines is dependent on the development of suitable chromatographic procedures to successfully purify genetic vectors, such as plasmid DNA. Human Papillomavirus is associated with the development of tumours due to the oncogenic power of E6 and E7 proteins, produced by this virus. The supercoiled HPV-16 E6/E7 plasmid-based vaccine was recently purified with the arginine monolith, with 100% of purity, but only 39% of recovery was achieved. Therefore, the present study describes the application of experimental design tools, a newly explored methodology in preparative chromatography, in order to improve the supercoiled plasmid DNA recovery with the arginine monolith, maintaining the high purity degree. In addition, the importance and influence of pH in the pDNA retention to the arginine ligand was also demonstrated. The Composite Central Face design was validated and the recovery of the target molecule was successfully improved from 39% to 83.5%, with an outstanding increase of more than double, while maintaining 100% of purity.
Journal: Journal of Chromatography B - Volumes 978–979, 26 January 2015, Pages 145–150